open access

Vol 43, No 3 (2005)
Original paper
Submitted: 2011-12-19
Published online: 2011-08-22
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Immunohistochemical detection of PPARγ receptors in the human pituitary adenomas: correlation with PCNA

Katarzyna Winczyk, Marek Pawlikowski
Folia Histochem Cytobiol 2005;43(3):137-141.

open access

Vol 43, No 3 (2005)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-08-22

Abstract

The occurrence of peroxisome proliferator-activated receptors gamma (PPARγ) was investigated in 51 human pituitary adenomas and in 6 non-tumoral human pituitary tissue samples. Moreover, the correlation between PPARγ and the proliferating cells nuclear antigen (PCNA) - immunocytochemical proliferation marker was evaluated. The receptors and PCNA were detected by immunohistochemical methods using the polyclonal anti-PPARγ and the monoclonal anti-PCNA antibodies, respectively. PPARγ were found in all examined tissues. The mean percentage of cells with positive nuclear reaction was 3-fold higher in pituitary adenomas in comparison with non-tumoral pituitary tissues. The strongest expression of PPARγ was observed in somatotropinomas. Besides the nuclear reaction, which is typical for PPARγ, positive immunostaining was also observed in the cytoplasm. It was clearly stronger in pituitary adenomas than in non-tumoral pituitary tissues. A slight, statistically insignificant tendency towards negative correlation between PPARγ and PCNA was found in somatotropinomas, prolactinomas, corticotropinomas and gonadotropinomas. On the other hand, in null cell adenomas and "silent" corticotropinomas, a strong positve correlation between the expression of PPARγ and PCNA was observed. The strong expression of PPARγ in human pituitary adenomas and its possible involvement in control of cell proliferation in these tumors give a good reason for the attempts of their treatment with PPARγ ligands.

Abstract

The occurrence of peroxisome proliferator-activated receptors gamma (PPARγ) was investigated in 51 human pituitary adenomas and in 6 non-tumoral human pituitary tissue samples. Moreover, the correlation between PPARγ and the proliferating cells nuclear antigen (PCNA) - immunocytochemical proliferation marker was evaluated. The receptors and PCNA were detected by immunohistochemical methods using the polyclonal anti-PPARγ and the monoclonal anti-PCNA antibodies, respectively. PPARγ were found in all examined tissues. The mean percentage of cells with positive nuclear reaction was 3-fold higher in pituitary adenomas in comparison with non-tumoral pituitary tissues. The strongest expression of PPARγ was observed in somatotropinomas. Besides the nuclear reaction, which is typical for PPARγ, positive immunostaining was also observed in the cytoplasm. It was clearly stronger in pituitary adenomas than in non-tumoral pituitary tissues. A slight, statistically insignificant tendency towards negative correlation between PPARγ and PCNA was found in somatotropinomas, prolactinomas, corticotropinomas and gonadotropinomas. On the other hand, in null cell adenomas and "silent" corticotropinomas, a strong positve correlation between the expression of PPARγ and PCNA was observed. The strong expression of PPARγ in human pituitary adenomas and its possible involvement in control of cell proliferation in these tumors give a good reason for the attempts of their treatment with PPARγ ligands.
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Keywords

PPARγ; PCNA; Immunohistochemistry; Pituitary adenomas

About this article
Title

Immunohistochemical detection of PPARγ receptors in the human pituitary adenomas: correlation with PCNA

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 3 (2005)

Article type

Original paper

Pages

137-141

Published online

2011-08-22

Bibliographic record

Folia Histochem Cytobiol 2005;43(3):137-141.

Keywords

PPARγ
PCNA
Immunohistochemistry
Pituitary adenomas

Authors

Katarzyna Winczyk
Marek Pawlikowski

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