open access

Vol 43, No 3 (2005)
Original paper
Submitted: 2011-12-19
Published online: 2011-08-22
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The influence of photodynamic therapy on apoptosis in human melanoma cell line

J. Saczko, J. Kulbacka, A. Chwiłkowska, M. Drąg-Zalesińska, T. Wysocka, M. Ługowski, T. Banas´
Folia Histochem Cytobiol 2005;43(3):129-132.

open access

Vol 43, No 3 (2005)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-08-22

Abstract

Melanoma is the most severe of all skin cancers as it may grow rapidly and metastasize. The application of photodynamic therapy (PDT) opens new perspectives in treatment of this cancer. Numerous studies suggest that the exposure of tumor cells to PDT can lead to cell death via two separate processes: apoptosis or necrosis. The aim of this study was to assess in vitro photodynamic therapy which induces apoptosis in the human Beidegröm Melanoma (BM) cell line, using neutral comet assay. The cells were incubated with Photofrin II (15 μg/ml and 30 μg/ml) 4 h before and 3 h after irradiation for 5 or 10 min with the light intensity of 10 mW/cm2, using a lamp with red filter (632.8 nm). The percentage of apoptotic cells was significantly higher after PDT comparing to control cells. We observed 25% and 70% of apoptotic cells after shorter irradiation and treatment with 15 μg/ml and 30 μg/ml of Ph II, respectively. After longer irradiation, the respective values were 71.9% and 90%. The results suggest that induction of apoptosis is an important determinant of photodynamic sensitivity in the studied cell line and that some types of DNA damage are dependent on photosensitizer concentration and time of irradiation.

Abstract

Melanoma is the most severe of all skin cancers as it may grow rapidly and metastasize. The application of photodynamic therapy (PDT) opens new perspectives in treatment of this cancer. Numerous studies suggest that the exposure of tumor cells to PDT can lead to cell death via two separate processes: apoptosis or necrosis. The aim of this study was to assess in vitro photodynamic therapy which induces apoptosis in the human Beidegröm Melanoma (BM) cell line, using neutral comet assay. The cells were incubated with Photofrin II (15 μg/ml and 30 μg/ml) 4 h before and 3 h after irradiation for 5 or 10 min with the light intensity of 10 mW/cm2, using a lamp with red filter (632.8 nm). The percentage of apoptotic cells was significantly higher after PDT comparing to control cells. We observed 25% and 70% of apoptotic cells after shorter irradiation and treatment with 15 μg/ml and 30 μg/ml of Ph II, respectively. After longer irradiation, the respective values were 71.9% and 90%. The results suggest that induction of apoptosis is an important determinant of photodynamic sensitivity in the studied cell line and that some types of DNA damage are dependent on photosensitizer concentration and time of irradiation.
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Keywords

Photodynamic therapy; Neutral comet assay; Photofrin II; Melanoma; Cell culture

About this article
Title

The influence of photodynamic therapy on apoptosis in human melanoma cell line

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 3 (2005)

Article type

Original paper

Pages

129-132

Published online

2011-08-22

Bibliographic record

Folia Histochem Cytobiol 2005;43(3):129-132.

Keywords

Photodynamic therapy
Neutral comet assay
Photofrin II
Melanoma
Cell culture

Authors

J. Saczko
J. Kulbacka
A. Chwiłkowska
M. Drąg-Zalesińska
T. Wysocka
M. Ługowski
T. Banas´

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