open access

Vol 43, No 4 (2005)
Original paper
Submitted: 2011-12-19
Published online: 2005-12-31
Get Citation

Localization of pectins and Ca2+ ions in unpollinated and pollinated wet (Petunia hybrida Hort.) and dry (Haemanthus albiflos L.) stigma.

Elzbieta Bednarska, Marta Lenartowska, Lidia Niekraś
Folia Histochem Cytobiol 2005;43(4):249-259.

open access

Vol 43, No 4 (2005)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2005-12-31

Abstract

The subcellular localization of Ca2+ ions as well as esterified and deesterified pectins in unpollinated and pollinated wet (Petunia hybrida) and dry (Haemanthus albiflos) stigma was analyzed. Stigmas with different surfaces were found to differ in Ca2+ and pectin localization. In a wet Petunia hybrida stigma, Ca2+ ions were present in the exudate occurring in the intercellular spaces of secretory tissue before pollination. The exudate of an unpollinated stigma was the site of the localization of large amounts of deesterified pectins. Stigma penetration by pollen tubes induced the lysis of this category of pectins. The epidermal cells walls of the dry Haemanthus albiflos stigma before pollination lacked free and loosely bound Ca2+ ions. Pollination induced an accumulation of these ions in the apoplast of the stigma epidermal cells. In cells walls of an unpollinated stigma, mainly esterified pectins were present. Their deesterification took place after pollination at the site of pollen grain adhesion and then at the site of pollen tube growth. These results have shown that wet and dry stigmas differ in pectin metabolism and in the mechanism of forming a calcium environment at the site of pollen grain germination.

Abstract

The subcellular localization of Ca2+ ions as well as esterified and deesterified pectins in unpollinated and pollinated wet (Petunia hybrida) and dry (Haemanthus albiflos) stigma was analyzed. Stigmas with different surfaces were found to differ in Ca2+ and pectin localization. In a wet Petunia hybrida stigma, Ca2+ ions were present in the exudate occurring in the intercellular spaces of secretory tissue before pollination. The exudate of an unpollinated stigma was the site of the localization of large amounts of deesterified pectins. Stigma penetration by pollen tubes induced the lysis of this category of pectins. The epidermal cells walls of the dry Haemanthus albiflos stigma before pollination lacked free and loosely bound Ca2+ ions. Pollination induced an accumulation of these ions in the apoplast of the stigma epidermal cells. In cells walls of an unpollinated stigma, mainly esterified pectins were present. Their deesterification took place after pollination at the site of pollen grain adhesion and then at the site of pollen tube growth. These results have shown that wet and dry stigmas differ in pectin metabolism and in the mechanism of forming a calcium environment at the site of pollen grain germination.
Get Citation
About this article
Title

Localization of pectins and Ca2+ ions in unpollinated and pollinated wet (Petunia hybrida Hort.) and dry (Haemanthus albiflos L.) stigma.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 4 (2005)

Article type

Original paper

Pages

249-259

Published online

2005-12-31

Bibliographic record

Folia Histochem Cytobiol 2005;43(4):249-259.

Authors

Elzbieta Bednarska
Marta Lenartowska
Lidia Niekraś

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl