open access

Vol 43, No 4 (2005)
Original paper
Submitted: 2011-12-19
Published online: 2005-12-31
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Messenger RNA electroporation: an efficient tool in immunotherapy and stem cell research.

A Van Driessche, P Ponsaerts, D R Van Bockstaele, V F I Van Tendeloo, Z N Berneman
Folia Histochem Cytobiol 2005;43(4):213-216.

open access

Vol 43, No 4 (2005)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2005-12-31

Abstract

Over the last decades medicine has developed tremendously, but still many diseases are incurable. The last years, cellular (gene) therapy has become a hot topic in biomedical research for the potential treatment of cancer, AIDS and diseases involving cell loss or degeneration. Here, we will focus on two major areas within cellular therapy, cellular immunotherapy and stem cell therapy, that could benefit from the introduction of neo-expressed genes through mRNA electroporation for basic research as well as for clinical applications. For cellular immunotherapy, we will provide a state-of-the-art on loading antigen-presenting cells with antigens in the mRNA format for manipulation of T cell immunity. In the area of stem cell research, we will highlight current gene transfer methods into adult and embryonic stem cells and discuss the use of mRNA electroporation for controlling guided differentiation of stem cells into specialized cell lineages.

Abstract

Over the last decades medicine has developed tremendously, but still many diseases are incurable. The last years, cellular (gene) therapy has become a hot topic in biomedical research for the potential treatment of cancer, AIDS and diseases involving cell loss or degeneration. Here, we will focus on two major areas within cellular therapy, cellular immunotherapy and stem cell therapy, that could benefit from the introduction of neo-expressed genes through mRNA electroporation for basic research as well as for clinical applications. For cellular immunotherapy, we will provide a state-of-the-art on loading antigen-presenting cells with antigens in the mRNA format for manipulation of T cell immunity. In the area of stem cell research, we will highlight current gene transfer methods into adult and embryonic stem cells and discuss the use of mRNA electroporation for controlling guided differentiation of stem cells into specialized cell lineages.
Get Citation
About this article
Title

Messenger RNA electroporation: an efficient tool in immunotherapy and stem cell research.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 43, No 4 (2005)

Article type

Original paper

Pages

213-216

Published online

2005-12-31

Bibliographic record

Folia Histochem Cytobiol 2005;43(4):213-216.

Authors

A Van Driessche
P Ponsaerts
D R Van Bockstaele
V F I Van Tendeloo
Z N Berneman

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