Vol 44, No 2 (2006)
Original paper
Submitted: 2011-12-19
Published online: 2006-06-30
Localization of the DAZ gene expression in seminiferous tubules of patients with spermatogenic disorders.
Anna Szczerba, Anna Jankowska, Mirosław Andrusiewicz, Jerzy B Warchoł
Folia Histochem Cytobiol 2006;44(2):123-126.
Vol 44, No 2 (2006)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2006-06-30
Abstract
The research on the expression and mutations of DAZ and its homologues in human and other mammals suggests that protein products of these genes can mainly affect development of germinal cells. The aim of the present study was to analyze the expression of the DAZ gene in seminiferous tubules of six men with spermatogenic disorders (hypospermatogenesis and spermatogenic arrest). The results based on the RT-PCR IS technique demonstrated that the DAZ product was present only in some seminiferous tubules and the fluorescence intensity was different within individual tubules. The most intense fluorescence characterised the spermatogonia, especially these organised in small groups inside separate tubules. In the patients with spermatogenic arrest at the spermatocyte stage, the DAZ gene transcripts were also found in primary spermatocytes. However, the fluorescence intensity of primary spermatocytes, except the fluorescence of the spermatocytes localised upon the lumen, was weaker than the fluorescence of spermatogonia. The results of our study showed that DAZ gene activity seems to correspond to the proliferative activity of stem cells of germinal epithelium.
Abstract
The research on the expression and mutations of DAZ and its homologues in human and other mammals suggests that protein products of these genes can mainly affect development of germinal cells. The aim of the present study was to analyze the expression of the DAZ gene in seminiferous tubules of six men with spermatogenic disorders (hypospermatogenesis and spermatogenic arrest). The results based on the RT-PCR IS technique demonstrated that the DAZ product was present only in some seminiferous tubules and the fluorescence intensity was different within individual tubules. The most intense fluorescence characterised the spermatogonia, especially these organised in small groups inside separate tubules. In the patients with spermatogenic arrest at the spermatocyte stage, the DAZ gene transcripts were also found in primary spermatocytes. However, the fluorescence intensity of primary spermatocytes, except the fluorescence of the spermatocytes localised upon the lumen, was weaker than the fluorescence of spermatogonia. The results of our study showed that DAZ gene activity seems to correspond to the proliferative activity of stem cells of germinal epithelium.
Title
Localization of the DAZ gene expression in seminiferous tubules of patients with spermatogenic disorders.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 44, No 2 (2006)
Article type
Original paper
Pages
123-126
Published online
2006-06-30
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1655
Article views/downloads
1096
Bibliographic record
Folia Histochem Cytobiol 2006;44(2):123-126.
Authors
Anna Szczerba
Anna Jankowska
Mirosław Andrusiewicz
Jerzy B Warchoł
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