Vol 44, No 3 (2006)
Original paper
Submitted: 2011-12-19
Published online: 2006-09-19
Androgen receptor in early apoptotic follicles in the porcine ovary at pregnancy.
Maria Słomczyńska, Zbigniew Tabarowski, Małgorzata Duda, Małgorzata Burek, Katarzyna Knapczyk
Folia Histochem Cytobiol 2006;44(3):185-188.
Vol 44, No 3 (2006)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2006-09-19
Abstract
Localization of androgen receptor (AR) was investigated in ovarian follicles developing and undergoing atresia during pregnancy in the pig. Immunohistochemical staining was conducted on ovarian antral follicles isolated on different days of gestation: 10, 18, 32, 50, 70, and 90. Paraffin sections were also subjected to in situ DNA labeling. TUNEL staining revealed the presence of positive follicles on all days of pregnancy but the amount of atretic follicles increased with time. However, even on day 90 of gestation many follicles were normal, with no signs of atresia. In atretic follicles, apoptotic cells were localized predominantly in the granulosa while theca was much less affected. Atretic follicles with many apoptotic cells were negative for AR. Nuclear immunostaining for AR was positive in follicles with limited amount of apoptotic cells. The same relationship was observed in ovarian follicles isolated at various days of pregnancy.
Abstract
Localization of androgen receptor (AR) was investigated in ovarian follicles developing and undergoing atresia during pregnancy in the pig. Immunohistochemical staining was conducted on ovarian antral follicles isolated on different days of gestation: 10, 18, 32, 50, 70, and 90. Paraffin sections were also subjected to in situ DNA labeling. TUNEL staining revealed the presence of positive follicles on all days of pregnancy but the amount of atretic follicles increased with time. However, even on day 90 of gestation many follicles were normal, with no signs of atresia. In atretic follicles, apoptotic cells were localized predominantly in the granulosa while theca was much less affected. Atretic follicles with many apoptotic cells were negative for AR. Nuclear immunostaining for AR was positive in follicles with limited amount of apoptotic cells. The same relationship was observed in ovarian follicles isolated at various days of pregnancy.
Title
Androgen receptor in early apoptotic follicles in the porcine ovary at pregnancy.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 44, No 3 (2006)
Article type
Original paper
Pages
185-188
Published online
2006-09-19
Page views
1680
Article views/downloads
1027
Bibliographic record
Folia Histochem Cytobiol 2006;44(3):185-188.
Authors
Maria Słomczyńska
Zbigniew Tabarowski
Małgorzata Duda
Małgorzata Burek
Katarzyna Knapczyk