Vol 44, No 3 (2006)
Original paper
Submitted: 2011-12-19
Published online: 2006-09-19
SDF-1 alone and in co-operation with HGF regulates biology of human cervical carcinoma cells.
Marcin Majka, Justyna Drukala, Ewa Lesko, Marcin Wysoczynski, Alfred B Jenson, Mariusz Z Ratajczak
Folia Histochem Cytobiol 2006;44(3):155-164.
Vol 44, No 3 (2006)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2006-09-19
Abstract
Stromal Derived Factor-1 (SDF-1)-CXCR4 axis plays a pivotal role in biology and metastasis of several tumors. The aim of this study was to see if SDF-1 alone or in combination with Hepatocyte Growth Factor (HGF) affects biology of human cervical carcinoma (HCC) cells. We found that HCC cell lines investigated in our study highly express CXCR4 on their surface. CXCR4 was also expressed on tumor cells in tissue sections derived from cervical cancer patients. At the same time normal cervical epithelium was negative for CXCR4 expression what suggests a strong correlation between CXCR4 and malignant cell phenotype. Subsequently, we studied a potential role of the SDF-1-CXCR4 axis in HCC and noticed that SDF-1 (i) chemoattracted HCC cells, (ii) enhanced their scattering, (iii) stimulated nuclear localization of beta-catenins and upregulated their target gene cyclin D1 and (iv) at the molecular level induced calcium flux and activated RAS-MAPK, PI3-AKT and JAK-STAT pathways. SDF-1-mediated functions were additionally enhanced in the presence of HGF. Thus, our data show that the SDF-1-CXCR4 axis affects biology of HCC cells. Furthermore, we postulate that this axis might become a potential target to prevent progression of cervical cancer.
Abstract
Stromal Derived Factor-1 (SDF-1)-CXCR4 axis plays a pivotal role in biology and metastasis of several tumors. The aim of this study was to see if SDF-1 alone or in combination with Hepatocyte Growth Factor (HGF) affects biology of human cervical carcinoma (HCC) cells. We found that HCC cell lines investigated in our study highly express CXCR4 on their surface. CXCR4 was also expressed on tumor cells in tissue sections derived from cervical cancer patients. At the same time normal cervical epithelium was negative for CXCR4 expression what suggests a strong correlation between CXCR4 and malignant cell phenotype. Subsequently, we studied a potential role of the SDF-1-CXCR4 axis in HCC and noticed that SDF-1 (i) chemoattracted HCC cells, (ii) enhanced their scattering, (iii) stimulated nuclear localization of beta-catenins and upregulated their target gene cyclin D1 and (iv) at the molecular level induced calcium flux and activated RAS-MAPK, PI3-AKT and JAK-STAT pathways. SDF-1-mediated functions were additionally enhanced in the presence of HGF. Thus, our data show that the SDF-1-CXCR4 axis affects biology of HCC cells. Furthermore, we postulate that this axis might become a potential target to prevent progression of cervical cancer.
Title
SDF-1 alone and in co-operation with HGF regulates biology of human cervical carcinoma cells.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 44, No 3 (2006)
Article type
Original paper
Pages
155-164
Published online
2006-09-19
Page views
1219
Article views/downloads
1215
Bibliographic record
Folia Histochem Cytobiol 2006;44(3):155-164.
Authors
Marcin Majka
Justyna Drukala
Ewa Lesko
Marcin Wysoczynski
Alfred B Jenson
Mariusz Z Ratajczak
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