open access

Vol 44, No 4 (2006)
ORIGINAL PAPERS
Published online: 2007-01-16
Submitted: 2011-12-19
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Riboflavin content in autofluorescent earthworm coelomocytes is species-specific.

Barbara Płytycz, Joanna Homa, Beata Kozioł, Małgorzata Rózanowska, A John Morgan
Folia Histochem Cytobiol 2006;44(4):275-280.

open access

Vol 44, No 4 (2006)
ORIGINAL PAPERS
Published online: 2007-01-16
Submitted: 2011-12-19

Abstract

We have recently shown that a large proproportion of earthworm coelomocytes exhibit strong autofluorescence in some species (Dendrobaena veneta, Allolobophora chlorotica, Dendrodrilus rubidus, Eisenia fetida, and Octolasion spp.), while autofluorescent coelomocytes are very scarce in representatives of Lumbricus spp. and Aporrectodea spp. Riboflavin (vitamin B2) was identified as a major fluorophore in Eisenia jetida coelomocytes. The main aim of the present experiments was to quantify riboflavin content in autofluorescent coelomocytes (eleocytes) from several earthworm species through a combination of flow cytometric and spectrofluorometric measurements. Spectrofluorometry of coelomocyte lysates showed that riboflavin was non-detectable in the coelomocytes of Aporrectodea spp. and Lumbricus spp., but was a prominent constituent of lysates from species with autofluorescent eleocytes. In the latter case, riboflavin content was the highest in E. fetida, followed by Octolasion spp. > A. chlorotica > D. rubidus. The riboflavin content of coelomocytes correlates positively with eleocyte autofluorescence intensity measured by flow cytometry and visible with fluorescence microscopy.

Abstract

We have recently shown that a large proproportion of earthworm coelomocytes exhibit strong autofluorescence in some species (Dendrobaena veneta, Allolobophora chlorotica, Dendrodrilus rubidus, Eisenia fetida, and Octolasion spp.), while autofluorescent coelomocytes are very scarce in representatives of Lumbricus spp. and Aporrectodea spp. Riboflavin (vitamin B2) was identified as a major fluorophore in Eisenia jetida coelomocytes. The main aim of the present experiments was to quantify riboflavin content in autofluorescent coelomocytes (eleocytes) from several earthworm species through a combination of flow cytometric and spectrofluorometric measurements. Spectrofluorometry of coelomocyte lysates showed that riboflavin was non-detectable in the coelomocytes of Aporrectodea spp. and Lumbricus spp., but was a prominent constituent of lysates from species with autofluorescent eleocytes. In the latter case, riboflavin content was the highest in E. fetida, followed by Octolasion spp. > A. chlorotica > D. rubidus. The riboflavin content of coelomocytes correlates positively with eleocyte autofluorescence intensity measured by flow cytometry and visible with fluorescence microscopy.
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About this article
Title

Riboflavin content in autofluorescent earthworm coelomocytes is species-specific.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 44, No 4 (2006)

Pages

275-280

Published online

2007-01-16

Bibliographic record

Folia Histochem Cytobiol 2006;44(4):275-280.

Authors

Barbara Płytycz
Joanna Homa
Beata Kozioł
Małgorzata Rózanowska
A John Morgan

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