open access
Vol 44, No 4 (2006)
Original paper
Submitted: 2011-12-19
Published online: 2007-01-16
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Immunohistochemical localization of proliferating cell nuclear antigen (PCNA) in the pig ovary.
,
Folia Histochem Cytobiol 2006;44(4):269-274.
open access
Vol 44, No 4 (2006)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2007-01-16
Abstract
The aim of the study was to determine the expression of proliferating cell nuclear antigen protein (PCNA) in the pig ovary. The localization of PCNA was demonstrated in paraffin sections of pig ovarian tissue using primary mouse monoclonal anti-PCNA antibody. In primordial follicles, no remarkable staining for PCNA either in granulosa cells or in the oocytes was observed. In primary to secondary follicles, positive staining in oocytes and in some granulosa cells was detected. The advanced preantral and particularly actively growing small to large antral follicles showed extensive PCNA labeling in the layers of granulosa and theca cells and in the cumulus cells encircling the oocyte. PCNA labeling was expressed in nuclei of oocytes in preantral and small antral follicles. In atretic follicles, the level of PCNA protein expression was dependent on the stage of atresia. Follicles demonstrating advanced atresia showed only limited or no PCNA labeled granulosa and theca cells. The results of the study demonstrate that follicular growth and development in pig ovary may be effectively monitored by determining the granulosa cell expression of PCNA.
Abstract
The aim of the study was to determine the expression of proliferating cell nuclear antigen protein (PCNA) in the pig ovary. The localization of PCNA was demonstrated in paraffin sections of pig ovarian tissue using primary mouse monoclonal anti-PCNA antibody. In primordial follicles, no remarkable staining for PCNA either in granulosa cells or in the oocytes was observed. In primary to secondary follicles, positive staining in oocytes and in some granulosa cells was detected. The advanced preantral and particularly actively growing small to large antral follicles showed extensive PCNA labeling in the layers of granulosa and theca cells and in the cumulus cells encircling the oocyte. PCNA labeling was expressed in nuclei of oocytes in preantral and small antral follicles. In atretic follicles, the level of PCNA protein expression was dependent on the stage of atresia. Follicles demonstrating advanced atresia showed only limited or no PCNA labeled granulosa and theca cells. The results of the study demonstrate that follicular growth and development in pig ovary may be effectively monitored by determining the granulosa cell expression of PCNA.
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About this articleTitle
Immunohistochemical localization of proliferating cell nuclear antigen (PCNA) in the pig ovary.
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
269-274
Published online
2007-01-16
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Bibliographic record
Folia Histochem Cytobiol 2006;44(4):269-274.
Authors
Milan TomĂĄnek
Ewa Chronowska
