open access

Vol 45, No 1 (2007)
Original paper
Submitted: 2011-12-19
Published online: 2007-03-24
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Coexpression of CD1a, langerin and Birbeck's granules in Langerhans cell histiocytoses (LCH) in children: ultrastructural and immunocytochemical studies.

Piotr Dziegiel, Barbara Dolilńska-Krajewska, Malgorzata Dumańska, Jadwiga Wecławek, Michał Jeleń, Marzena Podhorska-Okołów, Ewa Jagoda, Magdalena Fic, Maciej Zabel
Folia Histochem Cytobiol 2007;45(1):21-25.

open access

Vol 45, No 1 (2007)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2007-03-24

Abstract

Langerhans cell histiocytoses (LCH) represent rare diseases of unclear etiology and pathogenesis. Most of the cases include children, 1 to 15 years of age, and various organs are involved (bones, skin, liver, lymph nodes, bone marrow and other). The diagnosis of LCH used to be established by biopsy of the inflamed tissue and demonstration of expression of markers specific for Langerhans cells: CD1a and langerin. The diagnosis can be ultimately confirmed by demonstration of Birbeck's granules in the electron microscopy. The present study was aimed at immunocytochemical demonstration, in the examined LCH material (skin, bones, lymph nodes), of the specific antigen expression and at comparing it with the presence of Birbeck's granules. In the examined 11 cases co-expression of CD1a with langerin and with the presence of Birbeck's granules was noted. Also in all examined biopsies the expression of S-100 protein on inflammatory cells was found. The results corroborate the usefulness of immunocytochemical studies on CD 1 a and langerin expression in diagnosis of LCH.

Abstract

Langerhans cell histiocytoses (LCH) represent rare diseases of unclear etiology and pathogenesis. Most of the cases include children, 1 to 15 years of age, and various organs are involved (bones, skin, liver, lymph nodes, bone marrow and other). The diagnosis of LCH used to be established by biopsy of the inflamed tissue and demonstration of expression of markers specific for Langerhans cells: CD1a and langerin. The diagnosis can be ultimately confirmed by demonstration of Birbeck's granules in the electron microscopy. The present study was aimed at immunocytochemical demonstration, in the examined LCH material (skin, bones, lymph nodes), of the specific antigen expression and at comparing it with the presence of Birbeck's granules. In the examined 11 cases co-expression of CD1a with langerin and with the presence of Birbeck's granules was noted. Also in all examined biopsies the expression of S-100 protein on inflammatory cells was found. The results corroborate the usefulness of immunocytochemical studies on CD 1 a and langerin expression in diagnosis of LCH.
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About this article
Title

Coexpression of CD1a, langerin and Birbeck's granules in Langerhans cell histiocytoses (LCH) in children: ultrastructural and immunocytochemical studies.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 45, No 1 (2007)

Article type

Original paper

Pages

21-25

Published online

2007-03-24

Bibliographic record

Folia Histochem Cytobiol 2007;45(1):21-25.

Authors

Piotr Dziegiel
Barbara Dolilńska-Krajewska
Malgorzata Dumańska
Jadwiga Wecławek
Michał Jeleń
Marzena Podhorska-Okołów
Ewa Jagoda
Magdalena Fic
Maciej Zabel

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