Vol 45, No 1 (2007)
Original paper
Published online: 2007-03-24

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CD40L and IL-4 stimulation of acute lymphoblastic leukemia cells results in upregulation of mRNA level of FLICE--an important component of apoptosis.

Włodzimierz łuczyński, Oksana Kowalczuk, Elibieta Iłendo, Anna Stasiak-Barmuta, Maryna Krawczuk-Rybak, Iwona Malinowska, Andrzej Kołtan, Tomasz Szczepalński, Igor Olejnik, Radosław Jaworowski, Lech Chyczewski, Michał Matysiak, Mariusz Wysocki, Danuta Sońta-Jakimczyk, Maria Wieczorek
Folia Histochem Cytobiol 2007;45(1):15-20.

Abstract

The use of cancer vaccines based on dendritic cells (DC) presenting tumor antigens can be a promising tool in the treatment of leukemia. The functional characteristics of leukemia derived DC is still to be elucidated. CD40 promotes survival, proliferation and differentiation of normal B cells. CD40 triggering was used to enhance the poor antigen-presenting capacity of leukemic B-cells. Since it is still unclear whether CD40 ligation drives neoplastic B-cells to apoptosis or not, we assessed the mRNA expression of FLICE, FAS, FADD and TRADD - important components of apoptosis machinery, using real-time PCR in acute lymphoblastic leukemia cells before and after CD40 and IL-4 stimulation. ALL cells stimulated with CD40L/IL-4 expressed dendritic cell phenotype at mRNA and protein levels (upregulation of main costimulatory and adhesion molecules noted in real-time RT PCR and flow cytometry); they also expressed higher amounts of mRNA for FLICE, TRADD and FADD after CD40L/IL-4 stimulation. However differences statistically significant comparing cells cultured with CD40L/IL-4 and medium alone regarded only FLICE. Concluding, we showed upregulation of important elements of apoptosis at mRNA level in ALL cells after CD40 ligation.

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