Vol 45, No 2 (2007)
Original paper
Published online: 2007-06-29
Vitreous humour as a potential DNA source for postmortem human identification.
Folia Histochem Cytobiol 2007;45(2):135-136.
Abstract
PURPOSE
The aim of this study was the assessment of vitreous humor as a potential DNA for forensic human postmortem identification.
MATERIAL AND METHODS
Vitreous humor samples were collected using two alternative approaches from 25 corpses of either sex during autopsies. DNA was extracted by standard organic method. Recovered DNA was quantitiated fluorometrically. AmpFlSTR SGM Plus kit and ABI 310 Genetic Analyzer (Applera) were used to obtain genetic profiles.
RESULTS
Different DNA yields were quantitated in vitreous body depending on cause of death and sampling approach.
CONCLUSION
Vitreous humor is a potential DNA for forensic human postmortem identification depending on a sampling method used.
The aim of this study was the assessment of vitreous humor as a potential DNA for forensic human postmortem identification.
MATERIAL AND METHODS
Vitreous humor samples were collected using two alternative approaches from 25 corpses of either sex during autopsies. DNA was extracted by standard organic method. Recovered DNA was quantitiated fluorometrically. AmpFlSTR SGM Plus kit and ABI 310 Genetic Analyzer (Applera) were used to obtain genetic profiles.
RESULTS
Different DNA yields were quantitated in vitreous body depending on cause of death and sampling approach.
CONCLUSION
Vitreous humor is a potential DNA for forensic human postmortem identification depending on a sampling method used.
