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Vitreous humour as a potential DNA source for postmortem human identification.
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Abstract
The aim of this study was the assessment of vitreous humor as a potential DNA for forensic human postmortem identification.
MATERIAL AND METHODS
Vitreous humor samples were collected using two alternative approaches from 25 corpses of either sex during autopsies. DNA was extracted by standard organic method. Recovered DNA was quantitiated fluorometrically. AmpFlSTR SGM Plus kit and ABI 310 Genetic Analyzer (Applera) were used to obtain genetic profiles.
RESULTS
Different DNA yields were quantitated in vitreous body depending on cause of death and sampling approach.
CONCLUSION
Vitreous humor is a potential DNA for forensic human postmortem identification depending on a sampling method used.
Abstract
The aim of this study was the assessment of vitreous humor as a potential DNA for forensic human postmortem identification.
MATERIAL AND METHODS
Vitreous humor samples were collected using two alternative approaches from 25 corpses of either sex during autopsies. DNA was extracted by standard organic method. Recovered DNA was quantitiated fluorometrically. AmpFlSTR SGM Plus kit and ABI 310 Genetic Analyzer (Applera) were used to obtain genetic profiles.
RESULTS
Different DNA yields were quantitated in vitreous body depending on cause of death and sampling approach.
CONCLUSION
Vitreous humor is a potential DNA for forensic human postmortem identification depending on a sampling method used.
About this articleTitle
Vitreous humour as a potential DNA source for postmortem human identification.
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
135-136
Published online
2007-06-29
Page views
1012
Article views/downloads
1950
Bibliographic record
Folia Histochem Cytobiol 2007;45(2):135-136.
Authors
Ireneusz Stołyszewski
Anna Niemcunowicz-Janica
Witold Pepiński
Magdalena Spólnicka
Renata Zbiec
Jerzy Janica
