open access

Vol 45, No 2 (2007)
ORIGINAL PAPERS
Published online: 2007-06-29
Submitted: 2011-12-19
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Localization of RNA transcription sites in insect oocytes using microinjections of 5-bromouridine 5'-triphosphate.

Dmitry Bogolyubov
Folia Histochem Cytobiol 2007;45(2):129-134.

open access

Vol 45, No 2 (2007)
ORIGINAL PAPERS
Published online: 2007-06-29
Submitted: 2011-12-19

Abstract

In the present study we used 5-bromouridine 5'-triphosphate (BrUTP) microinjections to localize the transcription sites in oocytes of insects with different types of the ovarium structure: panoistic, meroistic polytrophic, and meroistic telotrophic. We found that in an insect with panoistic ovaries (Acheta domesticus), oocyte nuclei maintain their transcription activity during the long period of oocyte growth. In insects with meroistic ovaries (Tenebrio molitor and Panorpa communis), early oocyte chromosomes were found to be transcriptionally active, and some transcription activity still persist while the karyosphere, a compact structure formed by all condensed oocyte chromosomes, begins to develop. At the latest stages of karyosphere development, no anti-Br-RNA signal was registered in the karyosphere.

Abstract

In the present study we used 5-bromouridine 5'-triphosphate (BrUTP) microinjections to localize the transcription sites in oocytes of insects with different types of the ovarium structure: panoistic, meroistic polytrophic, and meroistic telotrophic. We found that in an insect with panoistic ovaries (Acheta domesticus), oocyte nuclei maintain their transcription activity during the long period of oocyte growth. In insects with meroistic ovaries (Tenebrio molitor and Panorpa communis), early oocyte chromosomes were found to be transcriptionally active, and some transcription activity still persist while the karyosphere, a compact structure formed by all condensed oocyte chromosomes, begins to develop. At the latest stages of karyosphere development, no anti-Br-RNA signal was registered in the karyosphere.
Get Citation
About this article
Title

Localization of RNA transcription sites in insect oocytes using microinjections of 5-bromouridine 5'-triphosphate.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 45, No 2 (2007)

Pages

129-134

Published online

2007-06-29

Bibliographic record

Folia Histochem Cytobiol 2007;45(2):129-134.

Authors

Dmitry Bogolyubov

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