open access
Vol 45, No 2 (2007)
Original paper
Submitted: 2011-12-19
Published online: 2007-06-29
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Localization of RNA transcription sites in insect oocytes using microinjections of 5-bromouridine 5'-triphosphate.
Folia Histochem Cytobiol 2007;45(2):129-134.
open access
Vol 45, No 2 (2007)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2007-06-29
Abstract
In the present study we used 5-bromouridine 5'-triphosphate (BrUTP) microinjections to localize the transcription sites in oocytes of insects with different types of the ovarium structure: panoistic, meroistic polytrophic, and meroistic telotrophic. We found that in an insect with panoistic ovaries (Acheta domesticus), oocyte nuclei maintain their transcription activity during the long period of oocyte growth. In insects with meroistic ovaries (Tenebrio molitor and Panorpa communis), early oocyte chromosomes were found to be transcriptionally active, and some transcription activity still persist while the karyosphere, a compact structure formed by all condensed oocyte chromosomes, begins to develop. At the latest stages of karyosphere development, no anti-Br-RNA signal was registered in the karyosphere.
Abstract
In the present study we used 5-bromouridine 5'-triphosphate (BrUTP) microinjections to localize the transcription sites in oocytes of insects with different types of the ovarium structure: panoistic, meroistic polytrophic, and meroistic telotrophic. We found that in an insect with panoistic ovaries (Acheta domesticus), oocyte nuclei maintain their transcription activity during the long period of oocyte growth. In insects with meroistic ovaries (Tenebrio molitor and Panorpa communis), early oocyte chromosomes were found to be transcriptionally active, and some transcription activity still persist while the karyosphere, a compact structure formed by all condensed oocyte chromosomes, begins to develop. At the latest stages of karyosphere development, no anti-Br-RNA signal was registered in the karyosphere.
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About this articleTitle
Localization of RNA transcription sites in insect oocytes using microinjections of 5-bromouridine 5'-triphosphate.
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
129-134
Published online
2007-06-29
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Bibliographic record
Folia Histochem Cytobiol 2007;45(2):129-134.
Authors
Dmitry Bogolyubov
