Vol 45, No 2 (2007)
Original paper
Submitted: 2011-12-19
Published online: 2007-06-29
Basal cell subpopulation as putative human prostate carcinoma stem cells.
Aleksandar Kuzmanov, Soren Hayrabedyan, Milen Karaivanov, Krassimira Todorova
Folia Histochem Cytobiol 2007;45(2):75-80.
Vol 45, No 2 (2007)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2007-06-29
Abstract
The present study examines the expression of p63, glutathione S-transferase-pi (GSTP1) and alpha-methylacyl-CoAracemase (AMACR) in serial slices in proliferative inflammatory atrophy (PIA) in order to implicate that some of the basal cells are probably the putative human prostate carcinoma stem cells (PHPCSC). Archived tissue sections obtained after radical prostatectomy from cases (n=30) comprising of PIA were tested using immunohistochemistry with antibodies against AMACR (Dako), p63 and GSTP1 (Labvision) and visualized by biotin-streptavidin-peroxidase kit (DAKO LSAB 2 kit). Quantitative immunohistochemistry analysis (QIHC) of the studied antigen expression levels revealed that there are two populations of p63 basal cells. Type I basal cells had high AMACR, low GSTP1 and p63 expression. Type II basal cells had low AMACR, high GSTP1 and p63 expression. Therefore, we propose that the putative human prostate carcinoma stem cells probably reside within the population of type I basal cells.
Abstract
The present study examines the expression of p63, glutathione S-transferase-pi (GSTP1) and alpha-methylacyl-CoAracemase (AMACR) in serial slices in proliferative inflammatory atrophy (PIA) in order to implicate that some of the basal cells are probably the putative human prostate carcinoma stem cells (PHPCSC). Archived tissue sections obtained after radical prostatectomy from cases (n=30) comprising of PIA were tested using immunohistochemistry with antibodies against AMACR (Dako), p63 and GSTP1 (Labvision) and visualized by biotin-streptavidin-peroxidase kit (DAKO LSAB 2 kit). Quantitative immunohistochemistry analysis (QIHC) of the studied antigen expression levels revealed that there are two populations of p63 basal cells. Type I basal cells had high AMACR, low GSTP1 and p63 expression. Type II basal cells had low AMACR, high GSTP1 and p63 expression. Therefore, we propose that the putative human prostate carcinoma stem cells probably reside within the population of type I basal cells.
Title
Basal cell subpopulation as putative human prostate carcinoma stem cells.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 45, No 2 (2007)
Article type
Original paper
Pages
75-80
Published online
2007-06-29
Page views
1408
Article views/downloads
1313
Bibliographic record
Folia Histochem Cytobiol 2007;45(2):75-80.
Authors
Aleksandar Kuzmanov
Soren Hayrabedyan
Milen Karaivanov
Krassimira Todorova
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