open access

Vol 45, No 3 (2007)
Original paper
Submitted: 2011-12-19
Published online: 2007-10-24
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Macrophage-specific RAM11 monoclonal antibody cross-reacts with basal cells of stratified squamous epithelia.

Grzegorz J Lis, Jan A Litwin, Alicja Furgal-Borzych, Joanna Zarzecka, Tadeusz Cichocki
Folia Histochem Cytobiol 2007;45(3):229-232.

open access

Vol 45, No 3 (2007)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2007-10-24

Abstract

RAM11 is a mouse monoclonal anti-rabbit macrophage antibody recognizing connective tissue and vascular (atheromatous tissue) macrophages. This study demonstrates a cross-reaction of RAM11 with an unknown antigen in rabbit normal epithelial cells. Formalin-fixed, paraffin sections of the New Zealand White rabbit normal skin, oral mucosa, esophagus, small intestine and lung were immunostained with RAM11 antibody followed by goat anti-mouse Cy-3-conjugated antiglobulin. RAM11-positive immunofluorescence was observed in basal layer cells of stratified squamous epithelia (skin, oral mucosa, esophagus). No RAM11 immunostaining was found in any cells of simple (intestinal, bronchial) epithelia. These findings show that basal cells of stratified squamous keratinized and non-keratinized epithelia of the rabbit express an antigenic epitope which is common with that of macrophage antigen recognized by RAM11 monoclonal antibody.

Abstract

RAM11 is a mouse monoclonal anti-rabbit macrophage antibody recognizing connective tissue and vascular (atheromatous tissue) macrophages. This study demonstrates a cross-reaction of RAM11 with an unknown antigen in rabbit normal epithelial cells. Formalin-fixed, paraffin sections of the New Zealand White rabbit normal skin, oral mucosa, esophagus, small intestine and lung were immunostained with RAM11 antibody followed by goat anti-mouse Cy-3-conjugated antiglobulin. RAM11-positive immunofluorescence was observed in basal layer cells of stratified squamous epithelia (skin, oral mucosa, esophagus). No RAM11 immunostaining was found in any cells of simple (intestinal, bronchial) epithelia. These findings show that basal cells of stratified squamous keratinized and non-keratinized epithelia of the rabbit express an antigenic epitope which is common with that of macrophage antigen recognized by RAM11 monoclonal antibody.
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About this article
Title

Macrophage-specific RAM11 monoclonal antibody cross-reacts with basal cells of stratified squamous epithelia.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 45, No 3 (2007)

Article type

Original paper

Pages

229-232

Published online

2007-10-24

Bibliographic record

Folia Histochem Cytobiol 2007;45(3):229-232.

Authors

Grzegorz J Lis
Jan A Litwin
Alicja Furgal-Borzych
Joanna Zarzecka
Tadeusz Cichocki

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