open access

Vol 45, No 3 (2007)
Original paper
Submitted: 2011-12-19
Published online: 2007-10-24
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Analysis of expression of MHC class I molecules and TAP genes in malignant human cell lines.

Mariusz Kaczmarek, Magdalena Frydrychowicz, Blazej Rubis, Ewa Mizera-Nyczak, Edyta Nieruchalska, Jan Sikora, Elzbieta Kaczmarek
Folia Histochem Cytobiol 2007;45(3):205-214.

open access

Vol 45, No 3 (2007)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2007-10-24

Abstract

TAP proteins (transporters associated with antigen processing) take part in the transport of oligopeptides created in proteasomes from cytoplasm into endoplasmic reticulum. In the endoplasmic reticulum those oligopeptides are bound to MHC class I molecules and transported to the cell surface. TAP proteins consist of two subunits: TAP1 and TAP2. It has been previously shown that TAP protein expression can be decreased in malignant cells, followed by reduced protein expression or complete lack of MHC class I antigens on the cell surface. The aim of the study was to characterize of MHC class I protein expression and TAP mRNA synthesis in twenty human malignant tumor cell lines. MHC class I protein expression was examined by immunohistochemistry and flow cytometry. Expression of TAP genes was studied using RT-PCR and real-time PCR. All tested cell lines expressed MHC class I molecules. Flow cytometry showed different expression of MHC class I protein in tested cell lines. Molecular analysis revealed the presence of TAP1 and TAP2 gene transcripts in all cell lines examined. Quantitative real time PCR analysis showed differences of gene expression among cell lines tested.

Abstract

TAP proteins (transporters associated with antigen processing) take part in the transport of oligopeptides created in proteasomes from cytoplasm into endoplasmic reticulum. In the endoplasmic reticulum those oligopeptides are bound to MHC class I molecules and transported to the cell surface. TAP proteins consist of two subunits: TAP1 and TAP2. It has been previously shown that TAP protein expression can be decreased in malignant cells, followed by reduced protein expression or complete lack of MHC class I antigens on the cell surface. The aim of the study was to characterize of MHC class I protein expression and TAP mRNA synthesis in twenty human malignant tumor cell lines. MHC class I protein expression was examined by immunohistochemistry and flow cytometry. Expression of TAP genes was studied using RT-PCR and real-time PCR. All tested cell lines expressed MHC class I molecules. Flow cytometry showed different expression of MHC class I protein in tested cell lines. Molecular analysis revealed the presence of TAP1 and TAP2 gene transcripts in all cell lines examined. Quantitative real time PCR analysis showed differences of gene expression among cell lines tested.
Get Citation
About this article
Title

Analysis of expression of MHC class I molecules and TAP genes in malignant human cell lines.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 45, No 3 (2007)

Article type

Original paper

Pages

205-214

Published online

2007-10-24

Bibliographic record

Folia Histochem Cytobiol 2007;45(3):205-214.

Authors

Mariusz Kaczmarek
Magdalena Frydrychowicz
Blazej Rubis
Ewa Mizera-Nyczak
Edyta Nieruchalska
Jan Sikora
Elzbieta Kaczmarek

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