Vol 45, No 3 (2007)
Original paper
Submitted: 2011-12-19
Published online: 2007-10-24
Actin filament reorganization in HL-60 leukemia cell line after treatment with G-CSF and GM-CSF.
Alina Grzanka, Magdalena Izdebska, Anna Litwiniec, Dariusz Grzanka, Barbara Safiejko-Mroczka
Folia Histochem Cytobiol 2007;45(3):191-197.
Vol 45, No 3 (2007)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2007-10-24
Abstract
Currently, information regarding the influence of growth factors on the cytoskeleton, including G-CSF and GMCSF, remains limited. In the present study we show alterations in F-actin distribution and cell cycle progression in HL-60 promyelocytic leukemia cells, resulting from treatment with these cytokines in vitro. We found that both agents caused F-actin reorganization. Although multiple potential effects of various growth factors have been described previously, in our experimental conditions, we observed some rather subtle differences between the effects of G-CSF and GM-CSF on studied cells. The presence of these cytokines in the cell environment caused not only increased F-actin labeling in the cytoplasm, but also a weaker intensity of peripheral ring staining in comparison with control cells. In spite of the fact that HL60 cells exposed to G-CSF and GM-CSF contained different F-actin structures such as aggregates and F-actin network, the rate of actin polymerization was not significantly enhanced. Moreover, alterations were mainly related to considerable changes in the relative proportion of these different structures, what might be reflected by specific features of the differentiation process, with regard to the kind of stimulating factor used. Thus, reorganization of F-actin and other results obtained in our experimental conditions, might reflect unique characteristics of the differentiation process in HL-60 cells, involving low apoptosis frequency, the G1 to S phase transition in the cell cycle, as well as possible alternative ways of the cell death.
Abstract
Currently, information regarding the influence of growth factors on the cytoskeleton, including G-CSF and GMCSF, remains limited. In the present study we show alterations in F-actin distribution and cell cycle progression in HL-60 promyelocytic leukemia cells, resulting from treatment with these cytokines in vitro. We found that both agents caused F-actin reorganization. Although multiple potential effects of various growth factors have been described previously, in our experimental conditions, we observed some rather subtle differences between the effects of G-CSF and GM-CSF on studied cells. The presence of these cytokines in the cell environment caused not only increased F-actin labeling in the cytoplasm, but also a weaker intensity of peripheral ring staining in comparison with control cells. In spite of the fact that HL60 cells exposed to G-CSF and GM-CSF contained different F-actin structures such as aggregates and F-actin network, the rate of actin polymerization was not significantly enhanced. Moreover, alterations were mainly related to considerable changes in the relative proportion of these different structures, what might be reflected by specific features of the differentiation process, with regard to the kind of stimulating factor used. Thus, reorganization of F-actin and other results obtained in our experimental conditions, might reflect unique characteristics of the differentiation process in HL-60 cells, involving low apoptosis frequency, the G1 to S phase transition in the cell cycle, as well as possible alternative ways of the cell death.
Title
Actin filament reorganization in HL-60 leukemia cell line after treatment with G-CSF and GM-CSF.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 45, No 3 (2007)
Article type
Original paper
Pages
191-197
Published online
2007-10-24
Page views
1826
Article views/downloads
1610
Bibliographic record
Folia Histochem Cytobiol 2007;45(3):191-197.
Authors
Alina Grzanka
Magdalena Izdebska
Anna Litwiniec
Dariusz Grzanka
Barbara Safiejko-Mroczka