open access

Vol 45, No 4 (2007)
Original paper
Submitted: 2011-12-19
Published online: 2008-01-01
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Interchromatin granule clusters in vitellogenic oocytes of the flesh fly, Sarcophaga sp.

Dmitry Bogolyubov, Irina Stepanova
Folia Histochem Cytobiol 2007;45(4):401-403.

open access

Vol 45, No 4 (2007)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2008-01-01

Abstract

Insect oocyte nuclei contain different extrachromosomal nuclear bodies including Cajal bodies and interchromatin granule clusters (IGCs). In the present study, we describe IGC equivalents in the vitellogenic oocytes of the flesh fly, Sarcophaga sp. These structures were found to consist of 20-40-nm granules and also include the fibrillar areas of high and low electron density. Immunogold labeling electron microscopy revealed IGC marker protein SC35, Sm proteins, and trimethylguanosine cap of small nuclear (sn) RNAs in these bodies. Antibody against the non-phosphorylated RNA polymerase II selectively labeled the fibrillar areas of low electron density located inside the IGCs.

Abstract

Insect oocyte nuclei contain different extrachromosomal nuclear bodies including Cajal bodies and interchromatin granule clusters (IGCs). In the present study, we describe IGC equivalents in the vitellogenic oocytes of the flesh fly, Sarcophaga sp. These structures were found to consist of 20-40-nm granules and also include the fibrillar areas of high and low electron density. Immunogold labeling electron microscopy revealed IGC marker protein SC35, Sm proteins, and trimethylguanosine cap of small nuclear (sn) RNAs in these bodies. Antibody against the non-phosphorylated RNA polymerase II selectively labeled the fibrillar areas of low electron density located inside the IGCs.
Get Citation
About this article
Title

Interchromatin granule clusters in vitellogenic oocytes of the flesh fly, Sarcophaga sp.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 45, No 4 (2007)

Article type

Original paper

Pages

401-403

Published online

2008-01-01

Bibliographic record

Folia Histochem Cytobiol 2007;45(4):401-403.

Authors

Dmitry Bogolyubov
Irina Stepanova

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