open access

Vol 45, No 4 (2007)
ORIGINAL PAPERS
Published online: 2008-01-01
Submitted: 2011-12-19
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Effect of soil and water environment on typeability of PowerPlex Y (Promega) in selected tissue samples.

Anna Niemcunowicz-Janica, Witold Pepinski, Jacek Robert Janica, Malgorzata Skawronska, Jerzy Janica, Ewa Koc-Zorawska, Ireneusz Stolyszewski
Folia Histochem Cytobiol 2007;45(4):393-395.

open access

Vol 45, No 4 (2007)
ORIGINAL PAPERS
Published online: 2008-01-01
Submitted: 2011-12-19

Abstract

In cases of decomposed bodies Y chromosomal STR markers may be useful in identification of a male relative. The authors assessed typeability PowerPlex Y (Promega) loci in tissue material stored in water and soil environment. Tissue material was collected during autopsies of five persons aged 20-30 years with time of death determined within the limit of 14 hours. Heart muscle, liver and lung specimens were stored in pond water, sea water, sand and peat soil. DNA was extracted by organic method from tissue samples collected in 7-day intervals. Liver specimens were typeable in all PowerPlex Y loci within 100 days of storage in pond water with gradual decline at DYS392 in sea water. Heart muscle specimens stored in pond water exhibited allelic loss at DYS19, DYS385, DYS389II and DYS392, while all loci were typeable in sea water stored samples. For lung specimens allelic loss was noted throughout the profile. Storage of liver specimens in peat soil for more than 14 days resulted in allelic drop-out, and after 21 days no profiles were typeable. Heart muscle specimens were typeable in all PowerPlex Y systems after 35-day storage in sand, while allelic drop-out and subsequent lack of profiles were noted after 14 and 35 days respectively. Lung specimens stored in garden soil exhibited allelic drop-out and subsequent lack of profiles after 7 and 21 days, respectively. All PowerPlex Y loci were typeable in the latter material in sand up to day 35 with gradual decline of longer amplicons (DYS19, DYS385, DYS389II and DYS392).

Abstract

In cases of decomposed bodies Y chromosomal STR markers may be useful in identification of a male relative. The authors assessed typeability PowerPlex Y (Promega) loci in tissue material stored in water and soil environment. Tissue material was collected during autopsies of five persons aged 20-30 years with time of death determined within the limit of 14 hours. Heart muscle, liver and lung specimens were stored in pond water, sea water, sand and peat soil. DNA was extracted by organic method from tissue samples collected in 7-day intervals. Liver specimens were typeable in all PowerPlex Y loci within 100 days of storage in pond water with gradual decline at DYS392 in sea water. Heart muscle specimens stored in pond water exhibited allelic loss at DYS19, DYS385, DYS389II and DYS392, while all loci were typeable in sea water stored samples. For lung specimens allelic loss was noted throughout the profile. Storage of liver specimens in peat soil for more than 14 days resulted in allelic drop-out, and after 21 days no profiles were typeable. Heart muscle specimens were typeable in all PowerPlex Y systems after 35-day storage in sand, while allelic drop-out and subsequent lack of profiles were noted after 14 and 35 days respectively. Lung specimens stored in garden soil exhibited allelic drop-out and subsequent lack of profiles after 7 and 21 days, respectively. All PowerPlex Y loci were typeable in the latter material in sand up to day 35 with gradual decline of longer amplicons (DYS19, DYS385, DYS389II and DYS392).
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About this article
Title

Effect of soil and water environment on typeability of PowerPlex Y (Promega) in selected tissue samples.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 45, No 4 (2007)

Pages

393-395

Published online

2008-01-01

Bibliographic record

Folia Histochem Cytobiol 2007;45(4):393-395.

Authors

Anna Niemcunowicz-Janica
Witold Pepinski
Jacek Robert Janica
Malgorzata Skawronska
Jerzy Janica
Ewa Koc-Zorawska
Ireneusz Stolyszewski

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