Vol 45, No 4 (2007)
Original paper
Submitted: 2011-12-19
Published online: 2008-01-01
Glucocorticoid receptor beta splice variant expression in patients with high and low activity of systemic lupus erythematosus.
Piotr Piotrowski, Michal Burzynski, Margarita Lianeri, Magdalenaa Mostowska, Mariusz Wudarski, Hanna Chwalinska-Sadowska, Pawel P Jagodzinski
Folia Histochem Cytobiol 2007;45(4):339-342.
Vol 45, No 4 (2007)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2008-01-01
Abstract
The glucocorticoid receptor (GR) occurs mainly in two alternative splice variants encoding GRalpha and GRbeta. The GRbeta variant does not contain a GC binding domain and cannot mediate anti-inflammatory GC effects. Peripheral blood mononuclear cells (PBMCs) were isolated from venous whole blood of twelve patients with SLE. Ten of the SLE patients exhibited low disease activity while two patients displayed highly active stage of the disease. The quantitative analysis of GRalpha and GRbeta transcripts in PBMC was performed by reverse transcription and real-time quantitative PCR SYBR Green I system. The protein level of GRalpha and GRbeta isoforms in PBMCs was determined by western blotting analysis. We found that the two SLE patients with high disease activity exhibited significantly elevated GRbeta transcript levels and corresponding protein levels in PBMCs. These preliminary findings suggest that increased expression of GRbeta isoform may be associated with relatively more severe clinical presentation of SLE syndrome.
Abstract
The glucocorticoid receptor (GR) occurs mainly in two alternative splice variants encoding GRalpha and GRbeta. The GRbeta variant does not contain a GC binding domain and cannot mediate anti-inflammatory GC effects. Peripheral blood mononuclear cells (PBMCs) were isolated from venous whole blood of twelve patients with SLE. Ten of the SLE patients exhibited low disease activity while two patients displayed highly active stage of the disease. The quantitative analysis of GRalpha and GRbeta transcripts in PBMC was performed by reverse transcription and real-time quantitative PCR SYBR Green I system. The protein level of GRalpha and GRbeta isoforms in PBMCs was determined by western blotting analysis. We found that the two SLE patients with high disease activity exhibited significantly elevated GRbeta transcript levels and corresponding protein levels in PBMCs. These preliminary findings suggest that increased expression of GRbeta isoform may be associated with relatively more severe clinical presentation of SLE syndrome.
Title
Glucocorticoid receptor beta splice variant expression in patients with high and low activity of systemic lupus erythematosus.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 45, No 4 (2007)
Article type
Original paper
Pages
339-342
Published online
2008-01-01
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1221
Article views/downloads
1353
Bibliographic record
Folia Histochem Cytobiol 2007;45(4):339-342.
Authors
Piotr Piotrowski
Michal Burzynski
Margarita Lianeri
Magdalenaa Mostowska
Mariusz Wudarski
Hanna Chwalinska-Sadowska
Pawel P Jagodzinski