open access

Vol 45, No 4 (2007)
Original paper
Submitted: 2011-12-19
Published online: 2008-01-01
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The ImageStream System: a key step to a new era in imaging.

Ewa K Zuba-Surma, Magdalena Kucia, Ahmed Abdel-Latif, James W Lillard, Mariusz Z Ratajczak
Folia Histochem Cytobiol 2007;45(4):279-290.

open access

Vol 45, No 4 (2007)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2008-01-01

Abstract

The aim of this article is to provide a brief review about the ImageStream system a novel tool for multiparameter cell analysis in flow. The instrument integrates the features of flow cytometry and fluorescence microscopy combined with a modern methodology for image analysis. Similar to flow cytometry, ImageStream allows analysis of a large number of cells based on their fluorescence features and provides statistical analysis of these features. Additionally, ImageStream allows detailed morphometric cellular analysis based on acquired cellular images integrating various morphometric and photometric features of the examined cells. Simply stated, ImageStream system is an advanced flow cytometer acquiring both integrated fluorescence signals as well as high quality fluorescence images and allowing muliparameter analysis. The innovative features of the instrument offer new analytical capabilities and allow for a multitude of possible applications beyond the current means of flow cytometry. While this article summarizes basic information about the features of ImageStream and its applications based on the available literature and it also describes our own experience.

Abstract

The aim of this article is to provide a brief review about the ImageStream system a novel tool for multiparameter cell analysis in flow. The instrument integrates the features of flow cytometry and fluorescence microscopy combined with a modern methodology for image analysis. Similar to flow cytometry, ImageStream allows analysis of a large number of cells based on their fluorescence features and provides statistical analysis of these features. Additionally, ImageStream allows detailed morphometric cellular analysis based on acquired cellular images integrating various morphometric and photometric features of the examined cells. Simply stated, ImageStream system is an advanced flow cytometer acquiring both integrated fluorescence signals as well as high quality fluorescence images and allowing muliparameter analysis. The innovative features of the instrument offer new analytical capabilities and allow for a multitude of possible applications beyond the current means of flow cytometry. While this article summarizes basic information about the features of ImageStream and its applications based on the available literature and it also describes our own experience.
Get Citation
About this article
Title

The ImageStream System: a key step to a new era in imaging.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 45, No 4 (2007)

Article type

Original paper

Pages

279-290

Published online

2008-01-01

Bibliographic record

Folia Histochem Cytobiol 2007;45(4):279-290.

Authors

Ewa K Zuba-Surma
Magdalena Kucia
Ahmed Abdel-Latif
James W Lillard
Mariusz Z Ratajczak

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