open access

Vol 45, Supp. I (2007)
ORIGINAL PAPERS
Published online: 2008-04-15
Submitted: 2011-12-19
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Evaluation of protamines 1 and 2 transcript contents in spermatozoa from asthenozoospermic men.

Bartosz Kempisty, Magdalena Depa-Martynow, Margarita Lianeri, Piotr Jedrzejczak, Agnieszka Darul-Wasowicz, Paweł P Jagodzinski

open access

Vol 45, Supp. I (2007)
ORIGINAL PAPERS
Published online: 2008-04-15
Submitted: 2011-12-19

Abstract

During mammalian spermatogenesis, the chromatin structure undergoes substantial condensation. The key role in this process is played by protamines 1 and 2 (PRM1, PRM2). We attempted to compare the levels of PRM1 and PRM2 transcripts in mature spermatozoa of normospermic and asthenozoospermic men. Human ejaculates from normozoospermic (n=70) and asthenozoospermic (n=100) donors were purified by centrifugation through discontinuous Percoll density gradient. RNA was isolated from spermatozoa according to the ChomczyĂąski and Sacchi method, treated with DNase I, and reverse-transcribed into cDNA. Using reverse transcription and real-time quantitative polymerase chain reaction analysis, we found a reduction in the levels of PRM1 and PRM2 transcripts in spermatozoa from asthenozoospermic men, as compared to controls (P<0.001). Our findings indicate that a reduction in contents of PRM1 and PRM2 transcripts in spermatozoa may be linked with asthenozoospermia.

Abstract

During mammalian spermatogenesis, the chromatin structure undergoes substantial condensation. The key role in this process is played by protamines 1 and 2 (PRM1, PRM2). We attempted to compare the levels of PRM1 and PRM2 transcripts in mature spermatozoa of normospermic and asthenozoospermic men. Human ejaculates from normozoospermic (n=70) and asthenozoospermic (n=100) donors were purified by centrifugation through discontinuous Percoll density gradient. RNA was isolated from spermatozoa according to the ChomczyĂąski and Sacchi method, treated with DNase I, and reverse-transcribed into cDNA. Using reverse transcription and real-time quantitative polymerase chain reaction analysis, we found a reduction in the levels of PRM1 and PRM2 transcripts in spermatozoa from asthenozoospermic men, as compared to controls (P<0.001). Our findings indicate that a reduction in contents of PRM1 and PRM2 transcripts in spermatozoa may be linked with asthenozoospermia.
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About this article
Title

Evaluation of protamines 1 and 2 transcript contents in spermatozoa from asthenozoospermic men.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 45, Supp. I (2007)

Pages

109-113

Published online

2008-04-15

Authors

Bartosz Kempisty
Magdalena Depa-Martynow
Margarita Lianeri
Piotr Jedrzejczak
Agnieszka Darul-Wasowicz
Paweł P Jagodzinski

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