Vol 45, Supp. I (2007)
Original paper
Submitted: 2011-12-19
Published online: 2008-04-15
Evaluation of protamines 1 and 2 transcript contents in spermatozoa from asthenozoospermic men.
Bartosz Kempisty, Magdalena Depa-Martynow, Margarita Lianeri, Piotr Jedrzejczak, Agnieszka Darul-Wasowicz, Paweł P Jagodzinski
Vol 45, Supp. I (2007)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2008-04-15
Abstract
During mammalian spermatogenesis, the chromatin structure undergoes substantial condensation. The key role in this process is played by protamines 1 and 2 (PRM1, PRM2). We attempted to compare the levels of PRM1 and PRM2 transcripts in mature spermatozoa of normospermic and asthenozoospermic men. Human ejaculates from normozoospermic (n=70) and asthenozoospermic (n=100) donors were purified by centrifugation through discontinuous Percoll density gradient. RNA was isolated from spermatozoa according to the ChomczyĂąski and Sacchi method, treated with DNase I, and reverse-transcribed into cDNA. Using reverse transcription and real-time quantitative polymerase chain reaction analysis, we found a reduction in the levels of PRM1 and PRM2 transcripts in spermatozoa from asthenozoospermic men, as compared to controls (P<0.001). Our findings indicate that a reduction in contents of PRM1 and PRM2 transcripts in spermatozoa may be linked with asthenozoospermia.
Abstract
During mammalian spermatogenesis, the chromatin structure undergoes substantial condensation. The key role in this process is played by protamines 1 and 2 (PRM1, PRM2). We attempted to compare the levels of PRM1 and PRM2 transcripts in mature spermatozoa of normospermic and asthenozoospermic men. Human ejaculates from normozoospermic (n=70) and asthenozoospermic (n=100) donors were purified by centrifugation through discontinuous Percoll density gradient. RNA was isolated from spermatozoa according to the ChomczyĂąski and Sacchi method, treated with DNase I, and reverse-transcribed into cDNA. Using reverse transcription and real-time quantitative polymerase chain reaction analysis, we found a reduction in the levels of PRM1 and PRM2 transcripts in spermatozoa from asthenozoospermic men, as compared to controls (P<0.001). Our findings indicate that a reduction in contents of PRM1 and PRM2 transcripts in spermatozoa may be linked with asthenozoospermia.
Title
Evaluation of protamines 1 and 2 transcript contents in spermatozoa from asthenozoospermic men.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 45, Supp. I (2007)
Article type
Original paper
Pages
109-113
Published online
2008-04-15
Page views
1282
Article views/downloads
1720
Authors
Bartosz Kempisty
Magdalena Depa-Martynow
Margarita Lianeri
Piotr Jedrzejczak
Agnieszka Darul-Wasowicz
Paweł P Jagodzinski