Sperm DNA adducts impair fertilization during ICSI but not during IVF.

Stanisław HorĂĄk; Anita Olejek; Piotr Widłak

Vol 45, Supp. I (2007)

Original paper

Submitted: 2011-12-19

Published online: 2008-04-15

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Sperm DNA adducts impair fertilization during ICSI but not during IVF.

Stanisław HorĂĄk, Anita Olejek, Piotr Widłak

Vol 45, Supp. I (2007)

ORIGINAL PAPERS

Submitted: 2011-12-19

Published online: 2008-04-15

Abstract

Many studies emphasize the influence of the status of spermatozoal nucleus on fertilization, mainly with regard to DNA fragmentation. This study was undertaken to analyze the influence of DNA adducts content in spermatozoa on fertilization during assisted reproduction. Ovarian hyperstimulation, oocyte retrieval and laboratory work-up in 61 IVF (in vitro fertilization) and 118 ICSI (intracytoplasmic sperm injection) first cycles were performed according to the same protocol. Semen analysis was made according to WHO Manual (1999). DNA adducts assay in spermatozoa was performed by 32Ppostlabeling method. In total 331 fertilizable oocytes were obtained during IVF and 659 during ICSI. Both groups differed significantly by sperm count, motility and morphology but not by the concentration of DNA adducts in spermatozoa (0.0306 +/- 0.0217 in IVF versus 0.0373 +/- 0.0321 in ICSI). The fertilization rate during IVF was significantly influenced by sperm count (p=0.0002) and motility (p=0.0037) but not by DNA adducts concentration (p=0.30528), whereas during ICSI was positively influenced by sperm motility (p=0.04669) and negatively by DNA adducts concentration (p=0.00796). DNA adducts concentration in spermatozoa significantly negatively influences fertilization rate during ICSI, but not during IVF.