open access

Vol 46, No 1 (2008)
ORIGINAL PAPERS
Published online: 2008-02-26
Submitted: 2011-12-19
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The glial Gomori-positive material is sulfane sulpfur.

Zbigniew Srebro, Małgorzata Iciek, Piotr Sura, Marta Góralska
DOI: 10.2478/v10042-008-0010-y
·
Folia Histochem Cytobiol 2008;46(1):73-77.

open access

Vol 46, No 1 (2008)
ORIGINAL PAPERS
Published online: 2008-02-26
Submitted: 2011-12-19

Abstract

The Gomori-positive glia are periventricular astrocytes with abundant cytoplasmic granular material, predominantly occupying a periventricular site in the brain. These granular inclusions are strongly stained with chrome hematoxylin in the Gomori's method as well as exhibit red autofluorescence and non-enzymatic peroxidase activity. The glial Gomori-positive material (GGPM) granules are positive in the performic acid Alcian blue method indicating the presence of protein-bound sulfur, what has been shown by our previous studies. The number of cells containing glial Gomori-positive granules dropped after administration of cyanide and increased under the influence of sulfane sulfur donor (diallyl disulfide). This suggests, that sulfur of these granules is a sulfane sulfur, possibly in the form of protein-bound cysteine persulfide. Sulfane sulfur is labile, reactive sulfur atom covalently bound to another sulfur atom. In this paper we present evidence that GGPM exhibit affinity to cyanolysis and its stainability in Gomori's method is due to the presence of protein-bound sulfane sulfur. The biological role of the Gomori-positive glia connected with protective properties of sulfane sulfur has been discussed.

Abstract

The Gomori-positive glia are periventricular astrocytes with abundant cytoplasmic granular material, predominantly occupying a periventricular site in the brain. These granular inclusions are strongly stained with chrome hematoxylin in the Gomori's method as well as exhibit red autofluorescence and non-enzymatic peroxidase activity. The glial Gomori-positive material (GGPM) granules are positive in the performic acid Alcian blue method indicating the presence of protein-bound sulfur, what has been shown by our previous studies. The number of cells containing glial Gomori-positive granules dropped after administration of cyanide and increased under the influence of sulfane sulfur donor (diallyl disulfide). This suggests, that sulfur of these granules is a sulfane sulfur, possibly in the form of protein-bound cysteine persulfide. Sulfane sulfur is labile, reactive sulfur atom covalently bound to another sulfur atom. In this paper we present evidence that GGPM exhibit affinity to cyanolysis and its stainability in Gomori's method is due to the presence of protein-bound sulfane sulfur. The biological role of the Gomori-positive glia connected with protective properties of sulfane sulfur has been discussed.
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About this article
Title

The glial Gomori-positive material is sulfane sulpfur.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 46, No 1 (2008)

Pages

73-77

Published online

2008-02-26

DOI

10.2478/v10042-008-0010-y

Bibliographic record

Folia Histochem Cytobiol 2008;46(1):73-77.

Authors

Zbigniew Srebro
Małgorzata Iciek
Piotr Sura
Marta Góralska

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