open access

Vol 46, No 1 (2008)
Original paper
Submitted: 2011-12-19
Published online: 2008-02-26
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Comparison of gene expression of mitogenic kinin path in adherent and non-adherent CD 34-stem cells using oligonucleotide microarrays.

Rafał Stojko, Andrzej Witek, Joanna Głogowska, Urszula Mazurek, Grzegorz Chromy, Krzysztof Wilk, Lukasz Witek, Monika Bojdys-Szyndlar, Krzysztof Machaj, Zygmunt Pojda
DOI: 10.2478/v10042-008-0005-8
·
Folia Histochem Cytobiol 2008;46(1):45-50.

open access

Vol 46, No 1 (2008)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2008-02-26

Abstract

One of the more interesting cells present in the umbilical cord blood - as far as their potential clinical use is concerned - are stem cells not presenting the CD34 antigen. These are the pluripotential cells with their biological properties similar to mesenchymal stem cells, with the ability to differentiate into such tissue types as bone, cartilage, nervous (to some extent), glia and muscle. The authors compared the activity of genes coding the proteins in mitogenic signal paths activated by kinin receptors using oligonucleotide microarrays in adherent and non-adherent CD 34- cells derived from umbilical cord blood. In the linear regression model with a 95% prognosis area for differentiating genes outside this area, the following genes were selected: c-jun (present in 3 isoforms) and c-fos. The fos and jun genes create the AP-1 transcriptive factor which regulates the expression of genes taking part in numerous cellular processes, including the cell cycle and mitosis. The obtained results shed some light on the molecular processes behind the MSC proliferation and are a starting point for further studies on the mesenchymal stem cell biology.

Abstract

One of the more interesting cells present in the umbilical cord blood - as far as their potential clinical use is concerned - are stem cells not presenting the CD34 antigen. These are the pluripotential cells with their biological properties similar to mesenchymal stem cells, with the ability to differentiate into such tissue types as bone, cartilage, nervous (to some extent), glia and muscle. The authors compared the activity of genes coding the proteins in mitogenic signal paths activated by kinin receptors using oligonucleotide microarrays in adherent and non-adherent CD 34- cells derived from umbilical cord blood. In the linear regression model with a 95% prognosis area for differentiating genes outside this area, the following genes were selected: c-jun (present in 3 isoforms) and c-fos. The fos and jun genes create the AP-1 transcriptive factor which regulates the expression of genes taking part in numerous cellular processes, including the cell cycle and mitosis. The obtained results shed some light on the molecular processes behind the MSC proliferation and are a starting point for further studies on the mesenchymal stem cell biology.
Get Citation
About this article
Title

Comparison of gene expression of mitogenic kinin path in adherent and non-adherent CD 34-stem cells using oligonucleotide microarrays.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 46, No 1 (2008)

Article type

Original paper

Pages

45-50

Published online

2008-02-26

DOI

10.2478/v10042-008-0005-8

Bibliographic record

Folia Histochem Cytobiol 2008;46(1):45-50.

Authors

Rafał Stojko
Andrzej Witek
Joanna Głogowska
Urszula Mazurek
Grzegorz Chromy
Krzysztof Wilk
Lukasz Witek
Monika Bojdys-Szyndlar
Krzysztof Machaj
Zygmunt Pojda

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