open access

Vol 46, No 1 (2008)
Original paper
Submitted: 2011-12-19
Published online: 2008-02-26
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Application of primed in situ DNA synthesis (PRINS) with telomere human commercial kit in molecular cytogenetics of Equus caballus and Sus scrofa scrofa.

Maciej Wnuk, Monika Bugno, Ewa Slota
DOI: 10.2478/v10042-008-0012-9
·
Folia Histochem Cytobiol 2008;46(1):85-88.

open access

Vol 46, No 1 (2008)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2008-02-26

Abstract

Recently, molecular techniques have become an indispensable tools for cytogenetic research. Especially, development of in situ techniques made possible detection at the chromosomal level, genes as well as repetitive sequences like telomeres or the DNA component of telomeres. One of these methods is primed in situ DNA synthesis (PRINS) using an oligonucleotide primer complementary to the specific DNA sequence. In this report we described application of PRINS technique with telomere human commercial kit to telomere sequences identification. This commercial kit may be use to visualization of interstitial telomeric signal in pig genome. PRINS is attractive complement to FISH for detection of DNA repetitive sequences and displays lower level of non-specific hybridization than conventional FISH.

Abstract

Recently, molecular techniques have become an indispensable tools for cytogenetic research. Especially, development of in situ techniques made possible detection at the chromosomal level, genes as well as repetitive sequences like telomeres or the DNA component of telomeres. One of these methods is primed in situ DNA synthesis (PRINS) using an oligonucleotide primer complementary to the specific DNA sequence. In this report we described application of PRINS technique with telomere human commercial kit to telomere sequences identification. This commercial kit may be use to visualization of interstitial telomeric signal in pig genome. PRINS is attractive complement to FISH for detection of DNA repetitive sequences and displays lower level of non-specific hybridization than conventional FISH.
Get Citation
About this article
Title

Application of primed in situ DNA synthesis (PRINS) with telomere human commercial kit in molecular cytogenetics of Equus caballus and Sus scrofa scrofa.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 46, No 1 (2008)

Article type

Original paper

Pages

85-88

Published online

2008-02-26

DOI

10.2478/v10042-008-0012-9

Bibliographic record

Folia Histochem Cytobiol 2008;46(1):85-88.

Authors

Maciej Wnuk
Monika Bugno
Ewa Slota

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