open access

Vol 46, No 2 (2008)
ORIGINAL PAPERS
Published online: 2008-06-04
Submitted: 2011-12-19
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Naive helper T cells from BCG-vaccinated volunteers produce IFN-gamma and IL-5 to mycobacterial antigen-pulsed dendritic cells.

Magdalena Kowalewicz-Kulbat, Dominik Kaźmierczak, Stefan Donevski, Franck Biet, JoĂŤl Pestel, Wiesława Rudnicka
DOI: 10.2478/v10042-008-0023-6
·
Folia Histochem Cytobiol 2008;46(2):153-157.

open access

Vol 46, No 2 (2008)
ORIGINAL PAPERS
Published online: 2008-06-04
Submitted: 2011-12-19

Abstract

Mycobacterium bovis bacillus Calmette-GuĂŠrin (BCG) is a live vaccine that has been used in routine vaccination against tuberculosis for nearly 80 years. However, its efficacy is controversial. The failure of BCG vaccination may be at least partially explained by the induction of poor or inappropriate host responses. Dendritic cells (DCs) are likely to play a key role in the induction of immune response to mycobacteria by polarizing the reactivity of T lymphocytes toward a Th1 profile, contributing to the generation of protective cellular immunity against mycobacteria. In this study we aimed to investigate the production of Th1 and Th2 cytokines by naive CD4+ T cells to mycobacterial antigen-pulsed DCs in the group of young, healthy BCG vaccinated volunteers. The response of naive helper T cells was compared with the response of total blood lymphocytes. Our present results clearly showed that circulating naive CD45RA+CD4+ lymphocytes from BCG-vaccinated subjects can become effector helper cells producing IFN-gamma and IL-5 under the stimulation by autologous dendritic cells presenting mycobacterial protein antigen-PPD or infected with live M. bovis BCG bacilli.

Abstract

Mycobacterium bovis bacillus Calmette-GuĂŠrin (BCG) is a live vaccine that has been used in routine vaccination against tuberculosis for nearly 80 years. However, its efficacy is controversial. The failure of BCG vaccination may be at least partially explained by the induction of poor or inappropriate host responses. Dendritic cells (DCs) are likely to play a key role in the induction of immune response to mycobacteria by polarizing the reactivity of T lymphocytes toward a Th1 profile, contributing to the generation of protective cellular immunity against mycobacteria. In this study we aimed to investigate the production of Th1 and Th2 cytokines by naive CD4+ T cells to mycobacterial antigen-pulsed DCs in the group of young, healthy BCG vaccinated volunteers. The response of naive helper T cells was compared with the response of total blood lymphocytes. Our present results clearly showed that circulating naive CD45RA+CD4+ lymphocytes from BCG-vaccinated subjects can become effector helper cells producing IFN-gamma and IL-5 under the stimulation by autologous dendritic cells presenting mycobacterial protein antigen-PPD or infected with live M. bovis BCG bacilli.
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About this article
Title

Naive helper T cells from BCG-vaccinated volunteers produce IFN-gamma and IL-5 to mycobacterial antigen-pulsed dendritic cells.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 46, No 2 (2008)

Pages

153-157

Published online

2008-06-04

DOI

10.2478/v10042-008-0023-6

Bibliographic record

Folia Histochem Cytobiol 2008;46(2):153-157.

Authors

Magdalena Kowalewicz-Kulbat
Dominik Kaźmierczak
Stefan Donevski
Franck Biet
JoĂŤl Pestel
Wiesława Rudnicka

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