open access

Vol 46, No 2 (2008)
ORIGINAL PAPERS
Published online: 2008-06-04
Submitted: 2011-12-19
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Application of primary cell cultures of laryngeal carcinoma and laser scanning cytometry in the evaluation of tumor reactivity to cisplatinum.

Janusz Klatka, Roman Paduch, Piotr Pozarowski, Wioletta Pietruszewska, Krzysztof Kupisz, Piotr Trojanowski, Jacek Roliński
DOI: 10.2478/v10042-008-0024-5
·
Folia Histochem Cytobiol 2008;46(2):159-164.

open access

Vol 46, No 2 (2008)
ORIGINAL PAPERS
Published online: 2008-06-04
Submitted: 2011-12-19

Abstract

Unsatisfactory effects of treatment of laryngeal carcinoma patients stimulate the clinicians as well as researchers to develop new more effective treatment models and to find new reliable prognostic factors. The aim of the present study was the evaluation of the use of primary cell cultures of the laryngeal carcinoma and laser scanning cytometry (LSC) in the assessment of tumor reactivity to cisplatinum. Nineteen primary cultures of laryngeal carcinoma cells established from fragments of laryngeal carcinoma infiltrations were cultured with or without cisplatin, stained with monoclonal antibodies against P53 and BCL-2 proteins and analyzed by LSC. Cisplatin added to the culture medium leads to the significant increase of P53 expression and decrease of BCL-2 expression. Moreover, changes of P53 and BCL-2 expressions were significantly correlated. Our findings of apoptosis regulatory mechanisms could be useful in patient qualification for the chemotherapeutic follow-up treatment.

Abstract

Unsatisfactory effects of treatment of laryngeal carcinoma patients stimulate the clinicians as well as researchers to develop new more effective treatment models and to find new reliable prognostic factors. The aim of the present study was the evaluation of the use of primary cell cultures of the laryngeal carcinoma and laser scanning cytometry (LSC) in the assessment of tumor reactivity to cisplatinum. Nineteen primary cultures of laryngeal carcinoma cells established from fragments of laryngeal carcinoma infiltrations were cultured with or without cisplatin, stained with monoclonal antibodies against P53 and BCL-2 proteins and analyzed by LSC. Cisplatin added to the culture medium leads to the significant increase of P53 expression and decrease of BCL-2 expression. Moreover, changes of P53 and BCL-2 expressions were significantly correlated. Our findings of apoptosis regulatory mechanisms could be useful in patient qualification for the chemotherapeutic follow-up treatment.
Get Citation
About this article
Title

Application of primary cell cultures of laryngeal carcinoma and laser scanning cytometry in the evaluation of tumor reactivity to cisplatinum.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 46, No 2 (2008)

Pages

159-164

Published online

2008-06-04

DOI

10.2478/v10042-008-0024-5

Bibliographic record

Folia Histochem Cytobiol 2008;46(2):159-164.

Authors

Janusz Klatka
Roman Paduch
Piotr Pozarowski
Wioletta Pietruszewska
Krzysztof Kupisz
Piotr Trojanowski
Jacek Roliński

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