open access
Vol 46, No 3 (2008)
Original paper
Submitted: 2011-12-19
Published online: 2008-12-06
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The utilization of the climatic chamber to evaluate the influence of ambient conditions on endocrine, nervous and immune systems of rats.
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DOI: 10.2478/v10042-008-0047-y
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Folia Histochem Cytobiol 2008;46(3):253-256.
open access
Vol 46, No 3 (2008)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2008-12-06
Abstract
The adaptation of an organism to a change in environmental conditions is a complex and in some aspects a poorly understood physiological process. The activating influence of stress on the sympathetic nervous system, the hypothalamic - pituitary - adrenal axis and the suppression of TSH, LH, FSH release is well known. The interplay of communication between the endocrine and immune systems plays an essential role in modulating the response to stress related mediators. The basis of many contradictory and incoherent results of experiments is due to the various methodologies of creating changes in environmental conditions, the way of collecting blood samples which influence stress mediators, the case of assessing the influence of many factors on reproductive functions and the performance of experiments without synchronization with the reproductive cycle. The review will focus on the presentation of simple and repeatable methods of development of an adaptation stress to changed environmental conditions (temperature, oxygenation, humidity) and the technique of blood collection during hour-long estimation of interactions between the endocrine, nervous and immune systems. We would like to place emphasis on appropriate ways of performing experiments on female rats, with regards to the choice of a suitable phase of the reproductive cycle. Also on ways of anaesthesia and microsurgical techniques of vein catheterisation for repeated blood sampling. The performance of all phases of the experiment allow us to estimate only the influence of environmental conditions and eliminate interfering factors during the process of preparing animal for the experiment.
Abstract
The adaptation of an organism to a change in environmental conditions is a complex and in some aspects a poorly understood physiological process. The activating influence of stress on the sympathetic nervous system, the hypothalamic - pituitary - adrenal axis and the suppression of TSH, LH, FSH release is well known. The interplay of communication between the endocrine and immune systems plays an essential role in modulating the response to stress related mediators. The basis of many contradictory and incoherent results of experiments is due to the various methodologies of creating changes in environmental conditions, the way of collecting blood samples which influence stress mediators, the case of assessing the influence of many factors on reproductive functions and the performance of experiments without synchronization with the reproductive cycle. The review will focus on the presentation of simple and repeatable methods of development of an adaptation stress to changed environmental conditions (temperature, oxygenation, humidity) and the technique of blood collection during hour-long estimation of interactions between the endocrine, nervous and immune systems. We would like to place emphasis on appropriate ways of performing experiments on female rats, with regards to the choice of a suitable phase of the reproductive cycle. Also on ways of anaesthesia and microsurgical techniques of vein catheterisation for repeated blood sampling. The performance of all phases of the experiment allow us to estimate only the influence of environmental conditions and eliminate interfering factors during the process of preparing animal for the experiment.
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About this articleTitle
The utilization of the climatic chamber to evaluate the influence of ambient conditions on endocrine, nervous and immune systems of rats.
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
253-256
Published online
2008-12-06
Page views
1855
Article views/downloads
1551
DOI
10.2478/v10042-008-0047-y
Bibliographic record
Folia Histochem Cytobiol 2008;46(3):253-256.
Authors
Arkadiusz Baran
Grzegorz Jakiel
Grazyna Wójcik
