open access

Vol 46, No 3 (2008)
Original paper
Submitted: 2011-12-19
Published online: 2008-12-06
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Platelets augment respiratory burst in neutrophils activated by selected species of gram-positive or gram-negative bacteria.

Jacek Miedzobrodzki, Tomasz Panz, Przemysław M Płonka, Katarzyna Zajac, Joanna Dracz, Kamila Pytel, Łukasz Mateuszuk, Stefan Chłopicki
DOI: 10.2478/v10042-008-0052-1
·
Folia Histochem Cytobiol 2008;46(3):383-388.

open access

Vol 46, No 3 (2008)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2008-12-06

Abstract

Neutrophils and platelets circulate in blood system and play important physiological roles as part of immunological system. Neutrophils are the first line of host defense against various intruders, and platelets are satellite cells cooperating with other components of defense system. Recent studies report about the cooperation among these types of cells. We analyzed the effect of platelets on oxygen burst in neutrophils triggered by Staphylococcus aureus and Escherichia coli bacteria in vitro. The effect of platelets on oxygen burst in neutrophils was measured by luminol enhanced chemiluminescence. Opsonized and non-opsonized bacteria were used as activators. Activation of neutrophils with live non-opsonized and opsonized bacteria in the presence of platelets increased the oxygen burst as compared to the same system without platelets. The gram-positive bacteria (Staphylococcus aureus) were causing higher activation than gram-negative bacteria (Escherichia coli). This work demonstrate that platelets potentate the response of neutrophils augmenting their respiratory burst in vitro when triggered by bacteria.

Abstract

Neutrophils and platelets circulate in blood system and play important physiological roles as part of immunological system. Neutrophils are the first line of host defense against various intruders, and platelets are satellite cells cooperating with other components of defense system. Recent studies report about the cooperation among these types of cells. We analyzed the effect of platelets on oxygen burst in neutrophils triggered by Staphylococcus aureus and Escherichia coli bacteria in vitro. The effect of platelets on oxygen burst in neutrophils was measured by luminol enhanced chemiluminescence. Opsonized and non-opsonized bacteria were used as activators. Activation of neutrophils with live non-opsonized and opsonized bacteria in the presence of platelets increased the oxygen burst as compared to the same system without platelets. The gram-positive bacteria (Staphylococcus aureus) were causing higher activation than gram-negative bacteria (Escherichia coli). This work demonstrate that platelets potentate the response of neutrophils augmenting their respiratory burst in vitro when triggered by bacteria.
Get Citation
About this article
Title

Platelets augment respiratory burst in neutrophils activated by selected species of gram-positive or gram-negative bacteria.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 46, No 3 (2008)

Article type

Original paper

Pages

383-388

Published online

2008-12-06

DOI

10.2478/v10042-008-0052-1

Bibliographic record

Folia Histochem Cytobiol 2008;46(3):383-388.

Authors

Jacek Miedzobrodzki
Tomasz Panz
Przemysław M Płonka
Katarzyna Zajac
Joanna Dracz
Kamila Pytel
Łukasz Mateuszuk
Stefan Chłopicki

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