open access

Vol 46, No 3 (2008)
ORIGINAL PAPERS
Published online: 2008-12-06
Submitted: 2011-12-19
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Aggregation formation mediated anoikis resistance of BEL7402 hepatoma cells.

Zhiyong Zhang, Lihui Han, Lili Cao, Xiaohong Liang, Yugang Liu, Hua Liu, Juan Du, Zhonghua Qu, Changjun Zhu, Suxia Liu, Hui Li, Wensheng Sun
DOI: 10.2478/v10042-008-0042-3
·
Folia Histochem Cytobiol 2008;46(3):331-336.

open access

Vol 46, No 3 (2008)
ORIGINAL PAPERS
Published online: 2008-12-06
Submitted: 2011-12-19

Abstract

Anoikis resistance is the prerequisite of cancer cells metastasis. Elucidation of the mechanism of anoikis resistance remains a significant challenge. We reported here a model to mimic anoikis resistant process of hepatoma cells in vitro. Experimental results indicated cell to cell aggregation could mediate anoikis resistance of BEL7402 hepatoma cells. Further investigation of these aggregations indicated the biological properties changed greatly after the hepatoma cells lost their anchorage. Aggregation forming process could be separated into three distinct phases according to their biological characteristics, comprising of premature phase, mature phase and postmature phase. Mature phase aggregations have the premium state of cell viability and may mimic the metastatic cells in the circulating system. Biological properties of these three phases aggregations were studied in details including morphological alteration, cell viability and microarray expression profiles. It indicated there was a great upregulation of adhesion molecules during the process of aggregation formation and the cell to cell contact in the aggregation may be mediated independent of calcium involved adhesion pathway. This model might shed light on the anoikis resistance mechanism of hepatoma cells and help to develop new therapies that may target the anoikis resistant hepatoma cells in the metastasis process.

Abstract

Anoikis resistance is the prerequisite of cancer cells metastasis. Elucidation of the mechanism of anoikis resistance remains a significant challenge. We reported here a model to mimic anoikis resistant process of hepatoma cells in vitro. Experimental results indicated cell to cell aggregation could mediate anoikis resistance of BEL7402 hepatoma cells. Further investigation of these aggregations indicated the biological properties changed greatly after the hepatoma cells lost their anchorage. Aggregation forming process could be separated into three distinct phases according to their biological characteristics, comprising of premature phase, mature phase and postmature phase. Mature phase aggregations have the premium state of cell viability and may mimic the metastatic cells in the circulating system. Biological properties of these three phases aggregations were studied in details including morphological alteration, cell viability and microarray expression profiles. It indicated there was a great upregulation of adhesion molecules during the process of aggregation formation and the cell to cell contact in the aggregation may be mediated independent of calcium involved adhesion pathway. This model might shed light on the anoikis resistance mechanism of hepatoma cells and help to develop new therapies that may target the anoikis resistant hepatoma cells in the metastasis process.
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About this article
Title

Aggregation formation mediated anoikis resistance of BEL7402 hepatoma cells.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 46, No 3 (2008)

Pages

331-336

Published online

2008-12-06

DOI

10.2478/v10042-008-0042-3

Bibliographic record

Folia Histochem Cytobiol 2008;46(3):331-336.

Authors

Zhiyong Zhang
Lihui Han
Lili Cao
Xiaohong Liang
Yugang Liu
Hua Liu
Juan Du
Zhonghua Qu
Changjun Zhu
Suxia Liu
Hui Li
Wensheng Sun

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