open access

Vol 46, No 4 (2008)
Original Papers
Published online: 2009-01-15
Submitted: 2011-12-19
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Expression of HLA-G in patients with B-cell chronic lymphocytic leukemia (B-CLL).

K Giannopoulos, A Dmoszyńska, A Bojarska-Junak, M Schmitt, J Roliński
DOI: 10.2478/v10042-008-0072-x
·
Folia Histochem Cytobiol 2008;46(4):457-460.

open access

Vol 46, No 4 (2008)
Original Papers
Published online: 2009-01-15
Submitted: 2011-12-19

Abstract

The expression of HLA-G was reported in certain malignancies and its role in escaping from immunosurveillance in cancers was proposed since HLA-G is a nonconventional HLA class I molecule that protects fetus from immunorecognition during pregnancy. Recent studies proposed HLA-G as novel prognostic marker for patients with B-CLL. HLA-G was showed to bear even better prognostic information compared to Zeta-chain associated protein of 70kDa (ZAP-70) and CD38 although some other authors did not find HLA-G expression in CLL. Therefore in this study we characterized the expression of HLA-G on both RNA and protein level. In most of 20 B-CLL patients we were able to detect signal from HLA-G using flow cytometry analysis. The expression of HLA-G was confirmed on messenger level by real-time RT-PCR experiments. No correlation between HLA-G expression and expression of well established prognostic factors such as ZAP-70 and CD38 was detected. These results confirm that HLA-G is expressed on CLL leukemic cells. Furthermore the expression of HLA-G on CLL cells suggests that this molecule might be involved in escaping of CLL cells from immunosurveillance.

Abstract

The expression of HLA-G was reported in certain malignancies and its role in escaping from immunosurveillance in cancers was proposed since HLA-G is a nonconventional HLA class I molecule that protects fetus from immunorecognition during pregnancy. Recent studies proposed HLA-G as novel prognostic marker for patients with B-CLL. HLA-G was showed to bear even better prognostic information compared to Zeta-chain associated protein of 70kDa (ZAP-70) and CD38 although some other authors did not find HLA-G expression in CLL. Therefore in this study we characterized the expression of HLA-G on both RNA and protein level. In most of 20 B-CLL patients we were able to detect signal from HLA-G using flow cytometry analysis. The expression of HLA-G was confirmed on messenger level by real-time RT-PCR experiments. No correlation between HLA-G expression and expression of well established prognostic factors such as ZAP-70 and CD38 was detected. These results confirm that HLA-G is expressed on CLL leukemic cells. Furthermore the expression of HLA-G on CLL cells suggests that this molecule might be involved in escaping of CLL cells from immunosurveillance.
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About this article
Title

Expression of HLA-G in patients with B-cell chronic lymphocytic leukemia (B-CLL).

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 46, No 4 (2008)

Pages

457-460

Published online

2009-01-15

DOI

10.2478/v10042-008-0072-x

Bibliographic record

Folia Histochem Cytobiol 2008;46(4):457-460.

Authors

K Giannopoulos
A Dmoszyńska
A Bojarska-Junak
M Schmitt
J Roliński

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