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Vol 46, No 4 (2008)
Original paper
Submitted: 2011-12-19
Published online: 2009-01-15
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Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: a role of rab3B in secretion of GH through porosome.

Akira Matsuno, Johbu Itoh, Akiko Mizutani, Susumu Takekoshi, R Yoshiyuki Osamura, Hiroko Okinaga, Fuyuaki Ide, Satoru Miyawaki, Takeshi Uno, Shuichiro Asano, Junichi Tanaka, Hiroshi Nakaguchi, Mitsuyoshi Sasaki, Mineko Murakami
DOI: 10.2478/v10042-008-0069-5
·
Folia Histochem Cytobiol 2008;46(4):419-421.

open access

Vol 46, No 4 (2008)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2009-01-15

Abstract

Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome.

Abstract

Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome.

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Title

Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: a role of rab3B in secretion of GH through porosome.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 46, No 4 (2008)

Article type

Original paper

Pages

419-421

Published online

2009-01-15

Page views

2361

Article views/downloads

1829

DOI

10.2478/v10042-008-0069-5

Bibliographic record

Folia Histochem Cytobiol 2008;46(4):419-421.

Authors

Akira Matsuno
Johbu Itoh
Akiko Mizutani
Susumu Takekoshi
R Yoshiyuki Osamura
Hiroko Okinaga
Fuyuaki Ide
Satoru Miyawaki
Takeshi Uno
Shuichiro Asano
Junichi Tanaka
Hiroshi Nakaguchi
Mitsuyoshi Sasaki
Mineko Murakami

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