Vol 47, No 1 (2009)
Original paper
Submitted: 2011-12-19
Published online: 2009-05-08
The effect of cisplatin on human larynx carcinoma cell motility.
Roman Paduch, Wojciech Rzeski, Janusz Klatka
DOI: 10.2478/v10042-009-0022-2
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Folia Histochem Cytobiol 2009;47(1):75-79.
Vol 47, No 1 (2009)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2009-05-08
Abstract
Head and neck tumors are one of the major public health problem all over the world. Cellular response of larynx carcinoma to cisplatin (CDDP) chemotherapy occurs both in cell-interdependent and cell-autonomous pathways. In the first pathway, cytotoxic signal transduction is mediated via gap-junctional intercellular communication (GIJC). CDDP also influence tumor cell migration.The aim of this study was the analysis of the effect of CDDP (0.5 microg/ml and 1.5 microg/ml) on the gap-junction intercellular communication and motility, respectively, in two new cell cultures (RK33 and RK45) derived from human larynx carcinoma. The migration of RK45 cell line was slightly inhibited and RK33 not affected after the incubation with CDDP. Tumor cells incubation with CDDP resulted in farther LY migration through neighboring cells beyond monolayer wound than in control cultures.In conclusion, there is a relationship between intercellular communication via gap junctions and motility of laryngeal tumor cells after CDDP application.
Abstract
Head and neck tumors are one of the major public health problem all over the world. Cellular response of larynx carcinoma to cisplatin (CDDP) chemotherapy occurs both in cell-interdependent and cell-autonomous pathways. In the first pathway, cytotoxic signal transduction is mediated via gap-junctional intercellular communication (GIJC). CDDP also influence tumor cell migration.The aim of this study was the analysis of the effect of CDDP (0.5 microg/ml and 1.5 microg/ml) on the gap-junction intercellular communication and motility, respectively, in two new cell cultures (RK33 and RK45) derived from human larynx carcinoma. The migration of RK45 cell line was slightly inhibited and RK33 not affected after the incubation with CDDP. Tumor cells incubation with CDDP resulted in farther LY migration through neighboring cells beyond monolayer wound than in control cultures.In conclusion, there is a relationship between intercellular communication via gap junctions and motility of laryngeal tumor cells after CDDP application.
Title
The effect of cisplatin on human larynx carcinoma cell motility.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 47, No 1 (2009)
Article type
Original paper
Pages
75-79
Published online
2009-05-08
Page views
2099
Article views/downloads
1677
DOI
10.2478/v10042-009-0022-2
Bibliographic record
Folia Histochem Cytobiol 2009;47(1):75-79.
Authors
Roman Paduch
Wojciech Rzeski
Janusz Klatka