Vol 47, No 2 (2009)
Original paper
Submitted: 2011-12-19
Published online: 2009-12-10
Percentage of LFA-1+ and ICAM-1+ peripheral blood mononuclear cells in children and adolescents with type 1 diabetes does not distinguish patients with vascular complications.
Przemysław Pawłowski, Mirosława Urban, Anna Stasiak-Barmuta, Janusz Myśliwiec, Małgorzata Pawłowska
DOI: 10.2478/v10042-009-0030-2
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Folia Histochem Cytobiol 2009;47(2):243-247.
Vol 47, No 2 (2009)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2009-12-10
Abstract
There are only few studies evaluating lymphocytes activation in the diabetic vascular complications. ICAM-1/LFA-1 adhesion molecules not only participate in the lymphocyte T proliferation but also mediate leukocyte migration to the site of inflammation. We assess a relationship between the percentage of ICAM-1 and LFA-1 expressing PBMCs and the evolution of vascular complications in T1D in children and adolescents. The study was carried out on 60 children and adolescents with T1D (aged 9-20): (a) T1D lasting <5 years (n=20), (b) T1D lasting >5 years (n=20), without complications c) T1D lasting >5 years complicated with microalbuminuria, arterial hypertension, diabetic retinopathy (20 n). 20 healthy volunteers, age and sex matched constituted the control group. The expression of adhesion molecules was evaluated by using three-color flow cytometry. In children and adolescents with T1D <5 years, the percentage of ICAM-1+ and LFA-1+ PBMCs was decreased vs. controls (p<0.05 and p<0.001, respectively). Both in patients with T1D>5 years without vascular complications and in T1D with vascular disease the percentage of LFA-1+ T lymphocytes was significantly reduced in the peripheral blood (p<0.001 vs. healthy controls). In conclusion the percentage of LFA-1+ and ICAM-1+ PBMCs does not distinguish patients with vascular complications however decreased percentage of LFA-1+ PMBCs could serve as a nonspecific marker of the development of local inflammatory process in Type 1 diabetes.
Abstract
There are only few studies evaluating lymphocytes activation in the diabetic vascular complications. ICAM-1/LFA-1 adhesion molecules not only participate in the lymphocyte T proliferation but also mediate leukocyte migration to the site of inflammation. We assess a relationship between the percentage of ICAM-1 and LFA-1 expressing PBMCs and the evolution of vascular complications in T1D in children and adolescents. The study was carried out on 60 children and adolescents with T1D (aged 9-20): (a) T1D lasting <5 years (n=20), (b) T1D lasting >5 years (n=20), without complications c) T1D lasting >5 years complicated with microalbuminuria, arterial hypertension, diabetic retinopathy (20 n). 20 healthy volunteers, age and sex matched constituted the control group. The expression of adhesion molecules was evaluated by using three-color flow cytometry. In children and adolescents with T1D <5 years, the percentage of ICAM-1+ and LFA-1+ PBMCs was decreased vs. controls (p<0.05 and p<0.001, respectively). Both in patients with T1D>5 years without vascular complications and in T1D with vascular disease the percentage of LFA-1+ T lymphocytes was significantly reduced in the peripheral blood (p<0.001 vs. healthy controls). In conclusion the percentage of LFA-1+ and ICAM-1+ PBMCs does not distinguish patients with vascular complications however decreased percentage of LFA-1+ PMBCs could serve as a nonspecific marker of the development of local inflammatory process in Type 1 diabetes.
Title
Percentage of LFA-1+ and ICAM-1+ peripheral blood mononuclear cells in children and adolescents with type 1 diabetes does not distinguish patients with vascular complications.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 47, No 2 (2009)
Article type
Original paper
Pages
243-247
Published online
2009-12-10
Page views
1636
Article views/downloads
1940
DOI
10.2478/v10042-009-0030-2
Bibliographic record
Folia Histochem Cytobiol 2009;47(2):243-247.
Authors
Przemysław Pawłowski
Mirosława Urban
Anna Stasiak-Barmuta
Janusz Myśliwiec
Małgorzata Pawłowska
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