open access

Vol 47, No 2 (2009)
Original paper
Submitted: 2011-12-19
Published online: 2009-12-10
Get Citation

Mutations in the KRAS gene in ovarian tumors.

Bozena Dobrzycka, Sławomir J Terlikowski, Oksana Kowalczuk, Wiesława Niklińska, Lech Chyczewski, Marek Kulikowski
DOI: 10.2478/v10042-009-0039-6
·
Folia Histochem Cytobiol 2009;47(2):221-224.

open access

Vol 47, No 2 (2009)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2009-12-10

Abstract

RAS genes are the most frequently mutated oncogenes detected in human cancer. In this study we analyzed the presence of mutations at codon 12 of the KRAS gene in 78 women with ovarian tumor, including 64 invasive ovarian cancers and 14 borderline ovarian tumors, using an RFLP-PCR technique and we evaluated whether such alterations were associated with the selected clinicopathological parameters of the patients. KRAS codon 12 gene mutations were found in 6,2% of ovarian cancer tissue and in 14,3% of the borderline ovarian tumor. KRAS mutations were found with a significantly higher frequency in mucinous and borderline tumors compared to serous tumors (p<0,01). Mutation frequency was correlated with the histological type of tumor, but not with stage, grade or patients age.

Abstract

RAS genes are the most frequently mutated oncogenes detected in human cancer. In this study we analyzed the presence of mutations at codon 12 of the KRAS gene in 78 women with ovarian tumor, including 64 invasive ovarian cancers and 14 borderline ovarian tumors, using an RFLP-PCR technique and we evaluated whether such alterations were associated with the selected clinicopathological parameters of the patients. KRAS codon 12 gene mutations were found in 6,2% of ovarian cancer tissue and in 14,3% of the borderline ovarian tumor. KRAS mutations were found with a significantly higher frequency in mucinous and borderline tumors compared to serous tumors (p<0,01). Mutation frequency was correlated with the histological type of tumor, but not with stage, grade or patients age.
Get Citation
About this article
Title

Mutations in the KRAS gene in ovarian tumors.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 47, No 2 (2009)

Article type

Original paper

Pages

221-224

Published online

2009-12-10

DOI

10.2478/v10042-009-0039-6

Bibliographic record

Folia Histochem Cytobiol 2009;47(2):221-224.

Authors

Bozena Dobrzycka
Sławomir J Terlikowski
Oksana Kowalczuk
Wiesława Niklińska
Lech Chyczewski
Marek Kulikowski

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl