Vol 47, No 3 (2009)
Original paper
Submitted: 2011-12-19
Published online: 2010-02-19
Effect of arsenic trioxide (Trisenox) on actin organization in K-562 erythroleukemia cells.
Magdalena Izdebska, Alina Grzanka, Maciej Ostrowski, Agnieszka Zuryń, Dariusz Grzanka
DOI: 10.2478/v10042-009-0080-5
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Folia Histochem Cytobiol 2009;47(3):453-459.
Vol 47, No 3 (2009)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2010-02-19
Abstract
Actin is one of the cytoskeletal proteins that take part in many cellular processes. The aim of this study was to show the influence of Trisenox (arsenic trioxide), on the cytoplasmic and nuclear F-actin organization. Arsenic trioxide is the proapoptotic factor. Together with increasing doses, it caused the increase in the number of cells undergoing apoptosis. Under arsenic trioxide treatment, cytoplasmic and nuclear F-actin (polymerized form of G-actin) was found reorganized. It was transformed into granulated structures. In cytometer studies fluorescence intensity of cytoplasmic F-actin after ATO treatment decreasing urgently in comparison to control. The obtained results may suggest the involvement of F-actin in apoptosis, especially in chromatin reorganization.
Abstract
Actin is one of the cytoskeletal proteins that take part in many cellular processes. The aim of this study was to show the influence of Trisenox (arsenic trioxide), on the cytoplasmic and nuclear F-actin organization. Arsenic trioxide is the proapoptotic factor. Together with increasing doses, it caused the increase in the number of cells undergoing apoptosis. Under arsenic trioxide treatment, cytoplasmic and nuclear F-actin (polymerized form of G-actin) was found reorganized. It was transformed into granulated structures. In cytometer studies fluorescence intensity of cytoplasmic F-actin after ATO treatment decreasing urgently in comparison to control. The obtained results may suggest the involvement of F-actin in apoptosis, especially in chromatin reorganization.
Title
Effect of arsenic trioxide (Trisenox) on actin organization in K-562 erythroleukemia cells.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 47, No 3 (2009)
Article type
Original paper
Pages
453-459
Published online
2010-02-19
Page views
2184
Article views/downloads
1958
DOI
10.2478/v10042-009-0080-5
Bibliographic record
Folia Histochem Cytobiol 2009;47(3):453-459.
Authors
Magdalena Izdebska
Alina Grzanka
Maciej Ostrowski
Agnieszka Zuryń
Dariusz Grzanka