open access

Vol 47, No 3 (2009)
ORIGINAL PAPERS
Published online: 2010-02-19
Submitted: 2011-12-19
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Comparison of chromosome centromere topology in differentiating cells with myogenic potential.

Bartosz Mikołajczak, Ewa Wiland, Natalia Rozwadowska, Marek Rucinski, Tomasz Mietkiewski, Maciej Kurpisz
DOI: 10.2478/v10042-009-0037-8
·
Folia Histochem Cytobiol 2009;47(3):377-383.

open access

Vol 47, No 3 (2009)
ORIGINAL PAPERS
Published online: 2010-02-19
Submitted: 2011-12-19

Abstract

Chromosome territories (CT's) constitute the critical element of the intranuclear architecture. Position of these compartmentalized structures plays an important role in functioning of entire genome. Present study was to examine whether the centromeres position of chromosomes 4, X and Y can be changed during differentiation from myoblasts to myotubes. Topological analysis of these centromeres was based on two-dimensional fluorescent hybridization in situ (2D-FISH). During differentiation process the majority of X chromosome centromeres analyzed shifted to the peripheral part of a nucleus and similar phenomenon was observed with one of the chromosome 4 centromeres. Completely different tendency was noticed when investigating the location of the chromosome Y centromeres. Centromeres of this chromosome migrated to the centre of a nucleus. The results obtained demonstrated visible changes in chromosome topology along the myogenic stem cells differentiation.

Abstract

Chromosome territories (CT's) constitute the critical element of the intranuclear architecture. Position of these compartmentalized structures plays an important role in functioning of entire genome. Present study was to examine whether the centromeres position of chromosomes 4, X and Y can be changed during differentiation from myoblasts to myotubes. Topological analysis of these centromeres was based on two-dimensional fluorescent hybridization in situ (2D-FISH). During differentiation process the majority of X chromosome centromeres analyzed shifted to the peripheral part of a nucleus and similar phenomenon was observed with one of the chromosome 4 centromeres. Completely different tendency was noticed when investigating the location of the chromosome Y centromeres. Centromeres of this chromosome migrated to the centre of a nucleus. The results obtained demonstrated visible changes in chromosome topology along the myogenic stem cells differentiation.
Get Citation
About this article
Title

Comparison of chromosome centromere topology in differentiating cells with myogenic potential.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 47, No 3 (2009)

Pages

377-383

Published online

2010-02-19

DOI

10.2478/v10042-009-0037-8

Bibliographic record

Folia Histochem Cytobiol 2009;47(3):377-383.

Authors

Bartosz Mikołajczak
Ewa Wiland
Natalia Rozwadowska
Marek Rucinski
Tomasz Mietkiewski
Maciej Kurpisz

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