Comparison of chromosome centromere topology in differentiating cells with myogenic potential.

Bartosz Mikołajczak; Ewa Wiland; Natalia Rozwadowska; Marek Rucinski; Tomasz Mietkiewski; Maciej Kurpisz

doi:10.2478/v10042-009-0037-8

Vol 47, No 3 (2009)

Original paper

Submitted: 2011-12-19

Published online: 2010-02-19

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Comparison of chromosome centromere topology in differentiating cells with myogenic potential.

Bartosz Mikołajczak, Ewa Wiland, Natalia Rozwadowska, Marek Rucinski, Tomasz Mietkiewski, Maciej Kurpisz

DOI: 10.2478/v10042-009-0037-8

Folia Histochem Cytobiol 2009;47(3):377-383.

Vol 47, No 3 (2009)

ORIGINAL PAPERS

Submitted: 2011-12-19

Published online: 2010-02-19

Abstract

Chromosome territories (CT's) constitute the critical element of the intranuclear architecture. Position of these compartmentalized structures plays an important role in functioning of entire genome. Present study was to examine whether the centromeres position of chromosomes 4, X and Y can be changed during differentiation from myoblasts to myotubes. Topological analysis of these centromeres was based on two-dimensional fluorescent hybridization in situ (2D-FISH). During differentiation process the majority of X chromosome centromeres analyzed shifted to the peripheral part of a nucleus and similar phenomenon was observed with one of the chromosome 4 centromeres. Completely different tendency was noticed when investigating the location of the chromosome Y centromeres. Centromeres of this chromosome migrated to the centre of a nucleus. The results obtained demonstrated visible changes in chromosome topology along the myogenic stem cells differentiation.