open access

Vol 53, No 4 (2015)
Original paper
Submitted: 2015-08-07
Accepted: 2015-12-14
Published online: 2015-12-17
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Sperm parameters and DNA fragmentation of balanced chromosomal rearrangements carriers

Ewa Pastuszek, Jolanta Kiewisz, Patrycja M. Kulwikowska, Mariusz Lukaszuk, Krzysztof Lukaszuk
DOI: 10.5603/fhc.a2015.0032
·
Pubmed: 26691892
·
Folia Histochem Cytobiol 2015;53(4):314-321.

open access

Vol 53, No 4 (2015)
ORIGINAL PAPERS
Submitted: 2015-08-07
Accepted: 2015-12-14
Published online: 2015-12-17

Abstract

Introduction. Somatic chromosomal rearrangements that occur in infertile males are thought to be one of the major genetic factors influencing male infertility. The objective of this retrospective study was to evaluate sperm parameters in a group of patients with balanced translocations.

Material and methods. We analyzed semen of 84 balanced somatic translocation carriers [35 Robertsonian translocation (RT group) and 49 reciprocal translocation (RCT group)] and 57 men with normal karyotype (control group).

Semen samples were evaluated for sperm concentration, its motion parameters and vitality, round cell number (CASA) and DNA fragmentation index (TUNEL). Cytogenetic evaluation was also performed for each study participant.

Results. Sperm concentrations were lower when comparing the RT group to the control (p < 0.001) and RCT groups (p < 0.05). Occurrence of abnormal sperm concentration was more common among RT carriers (74.3%) than in the other groups (42.9% in RCT group and 28.1% in control group). The sperm progressive motility and vitality in RT carriers (21.53% and 62.17%) were lower than in control group (39.77% and 77.47%, p < 0.001, respectively) and RCT carriers (31.47% and 76.17%, p < 0.001, respectively). The RCT carriers and the control group did not differ in regard to sperm concentration, progressive sperm motility, motility grade D and sperm vitality. There were no significant differences in DNA fragmentation in carriers of both studied structural chro­mosomal rearrangements in comparison to subjects with normal karyotype.

Conclusions. RT carriers had significantly lower semen parameters in comparison to not only the subjects with normal karyotypes but also the RCT carriers.

Abstract

Introduction. Somatic chromosomal rearrangements that occur in infertile males are thought to be one of the major genetic factors influencing male infertility. The objective of this retrospective study was to evaluate sperm parameters in a group of patients with balanced translocations.

Material and methods. We analyzed semen of 84 balanced somatic translocation carriers [35 Robertsonian translocation (RT group) and 49 reciprocal translocation (RCT group)] and 57 men with normal karyotype (control group).

Semen samples were evaluated for sperm concentration, its motion parameters and vitality, round cell number (CASA) and DNA fragmentation index (TUNEL). Cytogenetic evaluation was also performed for each study participant.

Results. Sperm concentrations were lower when comparing the RT group to the control (p < 0.001) and RCT groups (p < 0.05). Occurrence of abnormal sperm concentration was more common among RT carriers (74.3%) than in the other groups (42.9% in RCT group and 28.1% in control group). The sperm progressive motility and vitality in RT carriers (21.53% and 62.17%) were lower than in control group (39.77% and 77.47%, p < 0.001, respectively) and RCT carriers (31.47% and 76.17%, p < 0.001, respectively). The RCT carriers and the control group did not differ in regard to sperm concentration, progressive sperm motility, motility grade D and sperm vitality. There were no significant differences in DNA fragmentation in carriers of both studied structural chro­mosomal rearrangements in comparison to subjects with normal karyotype.

Conclusions. RT carriers had significantly lower semen parameters in comparison to not only the subjects with normal karyotypes but also the RCT carriers.

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Keywords

Robertsonian translocation; reciprocal translocation; balanced chromosomal rearrangements; sperm parameters; DNA fragmentation

Supp./Additional Files (1)
Supplementary data: Structural autosomal aberrations among 84 respondents
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About this article
Title

Sperm parameters and DNA fragmentation of balanced chromosomal rearrangements carriers

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 53, No 4 (2015)

Article type

Original paper

Pages

314-321

Published online

2015-12-17

DOI

10.5603/fhc.a2015.0032

Pubmed

26691892

Bibliographic record

Folia Histochem Cytobiol 2015;53(4):314-321.

Keywords

Robertsonian translocation
reciprocal translocation
balanced chromosomal rearrangements
sperm parameters
DNA fragmentation

Authors

Ewa Pastuszek
Jolanta Kiewisz
Patrycja M. Kulwikowska
Mariusz Lukaszuk
Krzysztof Lukaszuk

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