open access

Vol 47, No 5 (2009)
Original paper
Published online: 2010-01-14
Submitted: 2011-12-19
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Correlation between ovarian steroidogenesis and beta-endorphin in the Lizard Uromastyx acanthinura: Immunohistochemical approach.

Sadjia Hammouche, ThĂŠrese Gernigon, Jean Marie Exbrayat
DOI: 10.2478/v10042-009-0050-y
·
Folia Histochem Cytobiol 2009;47(5):95-100.

open access

Vol 47, No 5 (2009)
ORIGINAL PAPERS
Published online: 2010-01-14
Submitted: 2011-12-19

Abstract

In Mammals, opioid peptides are involved in various physiological processes including the reproductive function. The knowledge of the distribution of beta-endorphin, one of opioid peptides in Reptiles ovaries is very limited. Therefore, the present study used the lizard ovarian follicles to further elucidate the role of this peptide in steroidogenesis. In Uromastyx acanthinura, the localization of both this peptide and sex steroid hormone was investigated by the immunohistochemical approach. This technique was used to evaluate the distribution of these substances and their relationship. The beta-endorphin is strongly distributed in the granulosa cells and oocyte cytoplasm of the previtellogenic follicles in sexually quiescent lizards (winter) when steroidogenesis was interrupted. In spring, the signal became weak, or even absent, in the vitellogenic and previtellogenic follicles. The granulosa cells of the previtellogenic follicles showed an important synthesis of 17beta-estradiol. Females that did not undergo in vitellogenesis in spring showed the same profile than quiescent females of winter. These findings represent the first evidence of the presence of beta-endorphin in the ovary of this lizard. The seasonal variations observed in the reproductive cycle suggest that this opioid peptide is involved in the modulation of seasonal steroidogenesis.

Abstract

In Mammals, opioid peptides are involved in various physiological processes including the reproductive function. The knowledge of the distribution of beta-endorphin, one of opioid peptides in Reptiles ovaries is very limited. Therefore, the present study used the lizard ovarian follicles to further elucidate the role of this peptide in steroidogenesis. In Uromastyx acanthinura, the localization of both this peptide and sex steroid hormone was investigated by the immunohistochemical approach. This technique was used to evaluate the distribution of these substances and their relationship. The beta-endorphin is strongly distributed in the granulosa cells and oocyte cytoplasm of the previtellogenic follicles in sexually quiescent lizards (winter) when steroidogenesis was interrupted. In spring, the signal became weak, or even absent, in the vitellogenic and previtellogenic follicles. The granulosa cells of the previtellogenic follicles showed an important synthesis of 17beta-estradiol. Females that did not undergo in vitellogenesis in spring showed the same profile than quiescent females of winter. These findings represent the first evidence of the presence of beta-endorphin in the ovary of this lizard. The seasonal variations observed in the reproductive cycle suggest that this opioid peptide is involved in the modulation of seasonal steroidogenesis.
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About this article
Title

Correlation between ovarian steroidogenesis and beta-endorphin in the Lizard Uromastyx acanthinura: Immunohistochemical approach.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 47, No 5 (2009)

Article type

Original paper

Pages

95-100

Published online

2010-01-14

DOI

10.2478/v10042-009-0050-y

Bibliographic record

Folia Histochem Cytobiol 2009;47(5):95-100.

Authors

Sadjia Hammouche
ThĂŠrese Gernigon
Jean Marie Exbrayat

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