Vol 48, No 1 (2010)
Original paper
Submitted: 2011-12-19
Published online: 2010-06-10
The mRNA expression of pro- and anti-inflammatory cytokines in T regulatory cells in children with type 1 diabetes.
Włodzimierz Łuczyński, Anna Stasiak-Barmuta, Agnieszka Juchniewicz, Natalia Wawrusiewicz-Kurylonek, Elzbieta Iłendo, Justyna Kos, Adam Kretowski, Maria Górska, Lech Chyczewski, Artur Bossowski
DOI: 10.2478/v10042-008-0113-5
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Folia Histochem Cytobiol 2010;48(1):93-100.
Vol 48, No 1 (2010)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2010-06-10
Abstract
Type 1 diabetes mellitus (T1DM) is caused by the autoimmune-mediated destruction of insulin-producing beta cells in the pancreas. T regulatory cells (Tregs) represent an active mechanism of suppressing autoreactive T cells that escape central tolerance. The aim of our study was to test the hypothesis that T regulatory cells express pro- and anti-inflammatory cytokines, elements of cytotoxicity and OX40/4-1BB molecules. The examined group consisted of 50 children with T1DM. Fifty two healthy individuals (control group) were enrolled into the study. A flow cytometric analysis of T-cell subpopulations was performed using the following markers: anti-CD3, anti-CD4, anti-CD25, anti-CD127, anti-CD134 and anti-CD137. Concurrently with the flow cytometric assessment of Tregs we separated CD4+CD25+CD127dim/- cells for further mRNA analysis. mRNA levels for transcription factor FoxP3, pro- and anti-inflammatory cytokines (interferon gamma, interleukin-2, interleukin-4, interleukin-10, transforming growth factor beta1 and tumor necrosis factor alpha), activatory molecules (OX40, 4-1BB) and elements of cytotoxicity (granzyme B, perforin 1) were determined by real-time PCR technique. We found no alterations in the frequency of CD4+CD25highCD127low cells between diabetic and control children. Treg cells expressed mRNA for pro- and anti-inflammatory cytokines. Lower OX40 and higher 4-1BB mRNA but not protein levels in Treg cells in diabetic patients compared to the healthy children were noted. Our observations confirm the presence of mRNA for pro- and anti-inflammatory cytokines in CD4+CD25+CD127dim/- cells in the peripheral blood of children with T1DM. Further studies with the goal of developing new strategies to potentiate Treg function in autoimmune diseases are warranted.
Abstract
Type 1 diabetes mellitus (T1DM) is caused by the autoimmune-mediated destruction of insulin-producing beta cells in the pancreas. T regulatory cells (Tregs) represent an active mechanism of suppressing autoreactive T cells that escape central tolerance. The aim of our study was to test the hypothesis that T regulatory cells express pro- and anti-inflammatory cytokines, elements of cytotoxicity and OX40/4-1BB molecules. The examined group consisted of 50 children with T1DM. Fifty two healthy individuals (control group) were enrolled into the study. A flow cytometric analysis of T-cell subpopulations was performed using the following markers: anti-CD3, anti-CD4, anti-CD25, anti-CD127, anti-CD134 and anti-CD137. Concurrently with the flow cytometric assessment of Tregs we separated CD4+CD25+CD127dim/- cells for further mRNA analysis. mRNA levels for transcription factor FoxP3, pro- and anti-inflammatory cytokines (interferon gamma, interleukin-2, interleukin-4, interleukin-10, transforming growth factor beta1 and tumor necrosis factor alpha), activatory molecules (OX40, 4-1BB) and elements of cytotoxicity (granzyme B, perforin 1) were determined by real-time PCR technique. We found no alterations in the frequency of CD4+CD25highCD127low cells between diabetic and control children. Treg cells expressed mRNA for pro- and anti-inflammatory cytokines. Lower OX40 and higher 4-1BB mRNA but not protein levels in Treg cells in diabetic patients compared to the healthy children were noted. Our observations confirm the presence of mRNA for pro- and anti-inflammatory cytokines in CD4+CD25+CD127dim/- cells in the peripheral blood of children with T1DM. Further studies with the goal of developing new strategies to potentiate Treg function in autoimmune diseases are warranted.
Title
The mRNA expression of pro- and anti-inflammatory cytokines in T regulatory cells in children with type 1 diabetes.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 48, No 1 (2010)
Article type
Original paper
Pages
93-100
Published online
2010-06-10
Page views
3285
Article views/downloads
2210
DOI
10.2478/v10042-008-0113-5
Bibliographic record
Folia Histochem Cytobiol 2010;48(1):93-100.
Authors
Włodzimierz Łuczyński
Anna Stasiak-Barmuta
Agnieszka Juchniewicz
Natalia Wawrusiewicz-Kurylonek
Elzbieta Iłendo
Justyna Kos
Adam Kretowski
Maria Górska
Lech Chyczewski
Artur Bossowski
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