open access

Vol 48, No 1 (2010)
ORIGINAL PAPERS
Published online: 2010-06-10
Submitted: 2011-12-19
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Expression of basal cell marker revealed by RAM11 antibody during epithelial regeneration in rabbits.

Grzegorz J Lis, Ewa Jasek, Jan A Litwin, Mariusz Gajda, Joanna Zarzecka, Tadeusz Cichocki
DOI: 10.2478/v10042-008-0087-3
·
Folia Histochem Cytobiol 2010;48(1):89-92.

open access

Vol 48, No 1 (2010)
ORIGINAL PAPERS
Published online: 2010-06-10
Submitted: 2011-12-19

Abstract

RAM11 is a mouse monoclonal anti-rabbit macrophage antibody recognizing connective tissue and vascular macrophages. Our previous report showed that RAM11 reacted with basal cells of stratified squamous epithelia of rabbit skin, oral mucosa and esophagus. The aim of the present study was to follow the appearance of RAM11 immunoreactivity in basal cells of regenerating oral epithelium in rabbits. No RAM11 immunostaining was observed in the regenerating epithelium examined on days 1 and 3 of wound healing. A weak immunofluorescence first appeared on day 7 in single basal cells and 32% of RAM11- positive basal cells were observed on day 14. These findings indicate that expression of the antigen recognized by RAM11 antibody is a transient event in the differentiation of oral keratinocytes which not always occurs during epithelial repair, although it is a constant feature of epithelial turnover in mature epithelium. Therefore this antigen can be regarded as basal cell marker only in mature stratified squamous epithelia.

Abstract

RAM11 is a mouse monoclonal anti-rabbit macrophage antibody recognizing connective tissue and vascular macrophages. Our previous report showed that RAM11 reacted with basal cells of stratified squamous epithelia of rabbit skin, oral mucosa and esophagus. The aim of the present study was to follow the appearance of RAM11 immunoreactivity in basal cells of regenerating oral epithelium in rabbits. No RAM11 immunostaining was observed in the regenerating epithelium examined on days 1 and 3 of wound healing. A weak immunofluorescence first appeared on day 7 in single basal cells and 32% of RAM11- positive basal cells were observed on day 14. These findings indicate that expression of the antigen recognized by RAM11 antibody is a transient event in the differentiation of oral keratinocytes which not always occurs during epithelial repair, although it is a constant feature of epithelial turnover in mature epithelium. Therefore this antigen can be regarded as basal cell marker only in mature stratified squamous epithelia.
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About this article
Title

Expression of basal cell marker revealed by RAM11 antibody during epithelial regeneration in rabbits.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 48, No 1 (2010)

Pages

89-92

Published online

2010-06-10

DOI

10.2478/v10042-008-0087-3

Bibliographic record

Folia Histochem Cytobiol 2010;48(1):89-92.

Authors

Grzegorz J Lis
Ewa Jasek
Jan A Litwin
Mariusz Gajda
Joanna Zarzecka
Tadeusz Cichocki

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