open access
Vol 48, No 1 (2010)
Original paper
Submitted: 2011-12-19
Published online: 2010-06-10
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Effect of maternal deprivation on the gonadotrophin-releasing hormone (GnRH) and GnRH-associated peptide neurobiology in lambs during the transition from infancy to prepuberty.
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DOI: 10.2478/v10042-008-0108-2
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Folia Histochem Cytobiol 2010;48(1):12-18.
open access
Vol 48, No 1 (2010)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2010-06-10
Abstract
Using morphological criteria we describe the effect of maternal deprivation on the gonadotrophin-releasing hormone (GnRH) and GnRH-associated peptide (GAP) of the GnRH prohormone (proGnRH) in the preoptic area (POA)-hypothalamus during the weaning period. The immunohistochemical GnRH- and GAP-neuroanatomy was investigated in female 12-week-old weanling and maternally deprived lambs and 15-week-old weaned lambs. The GnRH-immunoreactive (ir) nerve elements in the POA were more numerous in weanling and weaned lambs in comparison with maternally deprived lambs, whereas the nerve elements ir for GAP were numerous in weanlings and scarce in remaining lambs. In the hypothalamus, GnRH-ir fibers were more numerous in weaned lambs in comparison with others. Immunoreactive GnRH in the median eminence was scarce in weanlings and comparable greater in maternally deprived and weaned lambs. In contrast to ir GnRH, the GAP-ir fibers and nerve terminals in the hypothalamus and median eminence were numerous in weanlings and maternally deprived lambs and scarce in weaned lambs. In conclusion, maternal deprivation affects the intraneuronal locations involved in the maturation of GnRH from proGnRH in the POA-hypothalamus of weanlings. The described effect involves the increase in the GnRH posttranslational processing and terminal accumulation in the median eminence, which reflects the maturational increase from the low infantile terminal storage to the high prepubertal one.
Abstract
Using morphological criteria we describe the effect of maternal deprivation on the gonadotrophin-releasing hormone (GnRH) and GnRH-associated peptide (GAP) of the GnRH prohormone (proGnRH) in the preoptic area (POA)-hypothalamus during the weaning period. The immunohistochemical GnRH- and GAP-neuroanatomy was investigated in female 12-week-old weanling and maternally deprived lambs and 15-week-old weaned lambs. The GnRH-immunoreactive (ir) nerve elements in the POA were more numerous in weanling and weaned lambs in comparison with maternally deprived lambs, whereas the nerve elements ir for GAP were numerous in weanlings and scarce in remaining lambs. In the hypothalamus, GnRH-ir fibers were more numerous in weaned lambs in comparison with others. Immunoreactive GnRH in the median eminence was scarce in weanlings and comparable greater in maternally deprived and weaned lambs. In contrast to ir GnRH, the GAP-ir fibers and nerve terminals in the hypothalamus and median eminence were numerous in weanlings and maternally deprived lambs and scarce in weaned lambs. In conclusion, maternal deprivation affects the intraneuronal locations involved in the maturation of GnRH from proGnRH in the POA-hypothalamus of weanlings. The described effect involves the increase in the GnRH posttranslational processing and terminal accumulation in the median eminence, which reflects the maturational increase from the low infantile terminal storage to the high prepubertal one.
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About this articleTitle
Effect of maternal deprivation on the gonadotrophin-releasing hormone (GnRH) and GnRH-associated peptide neurobiology in lambs during the transition from infancy to prepuberty.
Journal
Folia Histochemica et Cytobiologica
Issue
Article type
Original paper
Pages
12-18
Published online
2010-06-10
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2016
Article views/downloads
1749
DOI
10.2478/v10042-008-0108-2
Bibliographic record
Folia Histochem Cytobiol 2010;48(1):12-18.
Authors
Marta Wańkowska
Jolanta Polkowska
