open access

Vol 48, No 1 (2010)
Original paper
Published online: 2010-06-10
Submitted: 2011-12-19
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Effect of maternal deprivation on the gonadotrophin-releasing hormone (GnRH) and GnRH-associated peptide neurobiology in lambs during the transition from infancy to prepuberty.

Marta Wańkowska, Jolanta Polkowska
DOI: 10.2478/v10042-008-0108-2
·
Folia Histochem Cytobiol 2010;48(1):12-18.

open access

Vol 48, No 1 (2010)
ORIGINAL PAPERS
Published online: 2010-06-10
Submitted: 2011-12-19

Abstract

Using morphological criteria we describe the effect of maternal deprivation on the gonadotrophin-releasing hormone (GnRH) and GnRH-associated peptide (GAP) of the GnRH prohormone (proGnRH) in the preoptic area (POA)-hypothalamus during the weaning period. The immunohistochemical GnRH- and GAP-neuroanatomy was investigated in female 12-week-old weanling and maternally deprived lambs and 15-week-old weaned lambs. The GnRH-immunoreactive (ir) nerve elements in the POA were more numerous in weanling and weaned lambs in comparison with maternally deprived lambs, whereas the nerve elements ir for GAP were numerous in weanlings and scarce in remaining lambs. In the hypothalamus, GnRH-ir fibers were more numerous in weaned lambs in comparison with others. Immunoreactive GnRH in the median eminence was scarce in weanlings and comparable greater in maternally deprived and weaned lambs. In contrast to ir GnRH, the GAP-ir fibers and nerve terminals in the hypothalamus and median eminence were numerous in weanlings and maternally deprived lambs and scarce in weaned lambs. In conclusion, maternal deprivation affects the intraneuronal locations involved in the maturation of GnRH from proGnRH in the POA-hypothalamus of weanlings. The described effect involves the increase in the GnRH posttranslational processing and terminal accumulation in the median eminence, which reflects the maturational increase from the low infantile terminal storage to the high prepubertal one.

Abstract

Using morphological criteria we describe the effect of maternal deprivation on the gonadotrophin-releasing hormone (GnRH) and GnRH-associated peptide (GAP) of the GnRH prohormone (proGnRH) in the preoptic area (POA)-hypothalamus during the weaning period. The immunohistochemical GnRH- and GAP-neuroanatomy was investigated in female 12-week-old weanling and maternally deprived lambs and 15-week-old weaned lambs. The GnRH-immunoreactive (ir) nerve elements in the POA were more numerous in weanling and weaned lambs in comparison with maternally deprived lambs, whereas the nerve elements ir for GAP were numerous in weanlings and scarce in remaining lambs. In the hypothalamus, GnRH-ir fibers were more numerous in weaned lambs in comparison with others. Immunoreactive GnRH in the median eminence was scarce in weanlings and comparable greater in maternally deprived and weaned lambs. In contrast to ir GnRH, the GAP-ir fibers and nerve terminals in the hypothalamus and median eminence were numerous in weanlings and maternally deprived lambs and scarce in weaned lambs. In conclusion, maternal deprivation affects the intraneuronal locations involved in the maturation of GnRH from proGnRH in the POA-hypothalamus of weanlings. The described effect involves the increase in the GnRH posttranslational processing and terminal accumulation in the median eminence, which reflects the maturational increase from the low infantile terminal storage to the high prepubertal one.
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About this article
Title

Effect of maternal deprivation on the gonadotrophin-releasing hormone (GnRH) and GnRH-associated peptide neurobiology in lambs during the transition from infancy to prepuberty.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 48, No 1 (2010)

Article type

Original paper

Pages

12-18

Published online

2010-06-10

DOI

10.2478/v10042-008-0108-2

Bibliographic record

Folia Histochem Cytobiol 2010;48(1):12-18.

Authors

Marta Wańkowska
Jolanta Polkowska

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