open access

Vol 48, No 2 (2010)
Original paper
Submitted: 2011-12-19
Published online: 2010-08-03
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Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.

Elzbieta Czykier, Maciej Zabel, Jolanta Seidel
DOI: 10.2478/v10042-010-0003-5
·
Folia Histochem Cytobiol 2010;48(2):306-310.

open access

Vol 48, No 2 (2010)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2010-08-03

Abstract

The current study was inspired by the fact that since 2004 no report had appeared on the occurrence of this peptide in healthy parotid glands of humans and animals. The objective of the current study was to investigate the immunolocalization of PTHrP in the parotid gland of three male rodents: 6 common voles (Microtus arvalis, Pallas, 1779), 6 bank voles (Clethrionomys glareoulus, Schreber, 1780) and 6 white Swiss mice, as well as to find out any species differences in the distribution of this peptide in various types of cells of the parotid gland. Immunocytochemical reactions were performed using the ABC technique with specific rabbit antibodies against human PTHrP (34-53) (CALBIOCHEM), diluted 1:70 and 1:50. We observed positive PTHrP expression in the epithelial cells of the striated duct in all the three animal species. The expression was strong in white mouse and very strong in common vole and bank vole. In all the rodent species studied, the reaction for PTHrP was granular in nature and irregularly distributed in the cytoplasm, being definitely stronger at the base and weaker at the apex of the cells. The PTHrP expression was negative in the epithelium of the intercalated duct, interlobular duct, main excretory duct, as well as in the myoepithelial cells surrounding the excretory ducts or serous acini.

Abstract

The current study was inspired by the fact that since 2004 no report had appeared on the occurrence of this peptide in healthy parotid glands of humans and animals. The objective of the current study was to investigate the immunolocalization of PTHrP in the parotid gland of three male rodents: 6 common voles (Microtus arvalis, Pallas, 1779), 6 bank voles (Clethrionomys glareoulus, Schreber, 1780) and 6 white Swiss mice, as well as to find out any species differences in the distribution of this peptide in various types of cells of the parotid gland. Immunocytochemical reactions were performed using the ABC technique with specific rabbit antibodies against human PTHrP (34-53) (CALBIOCHEM), diluted 1:70 and 1:50. We observed positive PTHrP expression in the epithelial cells of the striated duct in all the three animal species. The expression was strong in white mouse and very strong in common vole and bank vole. In all the rodent species studied, the reaction for PTHrP was granular in nature and irregularly distributed in the cytoplasm, being definitely stronger at the base and weaker at the apex of the cells. The PTHrP expression was negative in the epithelium of the intercalated duct, interlobular duct, main excretory duct, as well as in the myoepithelial cells surrounding the excretory ducts or serous acini.
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About this article
Title

Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 48, No 2 (2010)

Article type

Original paper

Pages

306-310

Published online

2010-08-03

DOI

10.2478/v10042-010-0003-5

Bibliographic record

Folia Histochem Cytobiol 2010;48(2):306-310.

Authors

Elzbieta Czykier
Maciej Zabel
Jolanta Seidel

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