open access

Vol 48, No 2 (2010)
Original paper
Published online: 2010-08-03
Submitted: 2011-12-19
Get Citation

Changes in thickness of each layer of developing chicken cornea after administration of caffeine.

Monika Kujawa-Hadryś, Dariusz Tosik, Hieronim Bartel
DOI: 10.2478/v10042-010-0035-x
·
Folia Histochem Cytobiol 2010;48(2):273-277.

open access

Vol 48, No 2 (2010)
ORIGINAL PAPERS
Published online: 2010-08-03
Submitted: 2011-12-19

Abstract

The aim of the study was the presentation of changes in thickness of each layer of a developing cornea, that came into being under an influence of caffeine which was administered to chicken embryos. Research materials were 26 chicken embryos from breeding eggs that had been incubated. Breeding eggs were divided into two groups: control (n=30) in which Ringer liquid was given, and experimental (n=30) in which teratogenic dose of caffeine was administrated - 3.5 mg/egg. In 36th hour of incubation solutions were given with cannula through a hole in an egg shell directly onto amniotic membrane. After closing the hole with paraffin, eggs were put back into incubator. On 10th and 19th day of incubation corneas were taken for morphometric and morphological analysis. In experimental groups reduction of corneal thickness, thickening of corneal epithelium and corneal endothelium as well as Bowman's and Descemet's membranes, decrease of thickness of corneal stroma in comparison with the control group have been observed. Caffeine causes thickness changes of all layers and decreases the total thickness of a developing cornea.

Abstract

The aim of the study was the presentation of changes in thickness of each layer of a developing cornea, that came into being under an influence of caffeine which was administered to chicken embryos. Research materials were 26 chicken embryos from breeding eggs that had been incubated. Breeding eggs were divided into two groups: control (n=30) in which Ringer liquid was given, and experimental (n=30) in which teratogenic dose of caffeine was administrated - 3.5 mg/egg. In 36th hour of incubation solutions were given with cannula through a hole in an egg shell directly onto amniotic membrane. After closing the hole with paraffin, eggs were put back into incubator. On 10th and 19th day of incubation corneas were taken for morphometric and morphological analysis. In experimental groups reduction of corneal thickness, thickening of corneal epithelium and corneal endothelium as well as Bowman's and Descemet's membranes, decrease of thickness of corneal stroma in comparison with the control group have been observed. Caffeine causes thickness changes of all layers and decreases the total thickness of a developing cornea.
Get Citation
About this article
Title

Changes in thickness of each layer of developing chicken cornea after administration of caffeine.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 48, No 2 (2010)

Article type

Original paper

Pages

273-277

Published online

2010-08-03

DOI

10.2478/v10042-010-0035-x

Bibliographic record

Folia Histochem Cytobiol 2010;48(2):273-277.

Authors

Monika Kujawa-Hadryś
Dariusz Tosik
Hieronim Bartel

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl