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Vol 48, No 3 (2010)
Original paper
Published online: 2010-11-13
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Expression of MUC1 mucin in human umbilical vein endothelial cells (HUVEC).

Halina Porowska, Anna Paszkiewicz-Gadek, Joanna Wosek, Krzysztof Wnuczko, Małgorzata Rusak, Marek Szczepański
DOI: 10.2478/v10042-010-0027-x
·
2010;48(3):417-424.

open access

Vol 48, No 3 (2010)
ORIGINAL PAPERS
Published online: 2010-11-13

Abstract

Mucin 1 (MUC1) is a membrane-bound glycoprotein that is expressed by various epithelial cell types. MUC1 functions include modulation of cell adhesion, signal transduction, lubrication and hydration of epithelial surfaces, and their protection from infection. In this study we demonstrated that MUC1 is expressed in human umbilical vein endothelial cells (HUVECs) and could be released/shed from cellular membrane. MUC1 presence in these cells was verified using three methods: Western blotting, flow cytometry and metabolic labeling. We also showed that mucin expression is stimulated by proinflammatory cytokines: about a 2-fold increase was observed after TNF-Îą treatment and lower after IFN-Îł alone and in combination with TNF-Îą treatment. It can be assumed that the presence of MUC1 in endothelial cells may have an important role in the interactions with different cell types in physiological and pathological processes.

Abstract

Mucin 1 (MUC1) is a membrane-bound glycoprotein that is expressed by various epithelial cell types. MUC1 functions include modulation of cell adhesion, signal transduction, lubrication and hydration of epithelial surfaces, and their protection from infection. In this study we demonstrated that MUC1 is expressed in human umbilical vein endothelial cells (HUVECs) and could be released/shed from cellular membrane. MUC1 presence in these cells was verified using three methods: Western blotting, flow cytometry and metabolic labeling. We also showed that mucin expression is stimulated by proinflammatory cytokines: about a 2-fold increase was observed after TNF-Îą treatment and lower after IFN-Îł alone and in combination with TNF-Îą treatment. It can be assumed that the presence of MUC1 in endothelial cells may have an important role in the interactions with different cell types in physiological and pathological processes.
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About this article
Title

Expression of MUC1 mucin in human umbilical vein endothelial cells (HUVEC).

Journal

Issue

Vol 48, No 3 (2010)

Article type

Original paper

Pages

417-424

Published online

2010-11-13

Page views

2190

Article views/downloads

1944

DOI

10.2478/v10042-010-0027-x

Bibliographic record

2010;48(3):417-424.

Authors

Halina Porowska
Anna Paszkiewicz-Gadek
Joanna Wosek
Krzysztof Wnuczko
Małgorzata Rusak
Marek Szczepański

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