Vol 48, No 3 (2010)
Original paper
Submitted: 2011-12-19
Published online: 2010-11-13
Expression of MUC1 mucin in human umbilical vein endothelial cells (HUVEC).
Halina Porowska, Anna Paszkiewicz-Gadek, Joanna Wosek, Krzysztof Wnuczko, Małgorzata Rusak, Marek Szczepański
DOI: 10.2478/v10042-010-0027-x
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Folia Histochem Cytobiol 2010;48(3):417-424.
Vol 48, No 3 (2010)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2010-11-13
Abstract
Mucin 1 (MUC1) is a membrane-bound glycoprotein that is expressed by various epithelial cell types. MUC1 functions include modulation of cell adhesion, signal transduction, lubrication and hydration of epithelial surfaces, and their protection from infection. In this study we demonstrated that MUC1 is expressed in human umbilical vein endothelial cells (HUVECs) and could be released/shed from cellular membrane. MUC1 presence in these cells was verified using three methods: Western blotting, flow cytometry and metabolic labeling. We also showed that mucin expression is stimulated by proinflammatory cytokines: about a 2-fold increase was observed after TNF-Îą treatment and lower after IFN-Îł alone and in combination with TNF-Îą treatment. It can be assumed that the presence of MUC1 in endothelial cells may have an important role in the interactions with different cell types in physiological and pathological processes.
Abstract
Mucin 1 (MUC1) is a membrane-bound glycoprotein that is expressed by various epithelial cell types. MUC1 functions include modulation of cell adhesion, signal transduction, lubrication and hydration of epithelial surfaces, and their protection from infection. In this study we demonstrated that MUC1 is expressed in human umbilical vein endothelial cells (HUVECs) and could be released/shed from cellular membrane. MUC1 presence in these cells was verified using three methods: Western blotting, flow cytometry and metabolic labeling. We also showed that mucin expression is stimulated by proinflammatory cytokines: about a 2-fold increase was observed after TNF-Îą treatment and lower after IFN-Îł alone and in combination with TNF-Îą treatment. It can be assumed that the presence of MUC1 in endothelial cells may have an important role in the interactions with different cell types in physiological and pathological processes.
Title
Expression of MUC1 mucin in human umbilical vein endothelial cells (HUVEC).
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 48, No 3 (2010)
Article type
Original paper
Pages
417-424
Published online
2010-11-13
Page views
2456
Article views/downloads
2149
DOI
10.2478/v10042-010-0027-x
Bibliographic record
Folia Histochem Cytobiol 2010;48(3):417-424.
Authors
Halina Porowska
Anna Paszkiewicz-Gadek
Joanna Wosek
Krzysztof Wnuczko
Małgorzata Rusak
Marek Szczepański