open access

Vol 48, No 3 (2010)
ORIGINAL PAPERS
Published online: 2010-11-13
Submitted: 2011-12-19
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An immunohistochemical study of the pancreatic endocrine cells of the ddN mouse.

Hyeung Sik Lee, Jeong Hyun Chang, Sae Kwang Ku
DOI: 10.2478/v10042-010-0026-y
·
Folia Histochem Cytobiol 2010;48(3):387-393.

open access

Vol 48, No 3 (2010)
ORIGINAL PAPERS
Published online: 2010-11-13
Submitted: 2011-12-19

Abstract

The regional distribution and frequency of the pancreatic endocrine cells in the ddN mouse were studied using specific antisera against insulin, glucagon, somatostatin and human pancreatic polypeptide (hPP). In the pancreatic islets, most of insulin-immunoreactive (IR) cells were located in the central region, and glucagon-, somatostatin and hPP-IR cells were located in the peripheral region regardless of the lobe. In the splenic part, glucagon-IR cells were also located in the central regions, and more numerous somatostatin-IR cells were detected in the central regions as compared with the duo-denal part. hPP-IR cells were restricted to the peripheral regions in both lobes but more numerous cells were detected in the duodenal portion. In the exocrine parenchyma of the splenic lobe, only insulin- and glucagon-IR cells were detected but all four kinds of IR cells were observed in the duodenal portion. In addition, insulin and hPP-IR cells were also demonstrated in the pancreatic duct regions. In conclusion, some strain-dependent characteristic distributional patterns of pancreatic endocrine cells were found in the ddN mouse with somewhat different distributional patterns between the two pancreatic lobes.

Abstract

The regional distribution and frequency of the pancreatic endocrine cells in the ddN mouse were studied using specific antisera against insulin, glucagon, somatostatin and human pancreatic polypeptide (hPP). In the pancreatic islets, most of insulin-immunoreactive (IR) cells were located in the central region, and glucagon-, somatostatin and hPP-IR cells were located in the peripheral region regardless of the lobe. In the splenic part, glucagon-IR cells were also located in the central regions, and more numerous somatostatin-IR cells were detected in the central regions as compared with the duo-denal part. hPP-IR cells were restricted to the peripheral regions in both lobes but more numerous cells were detected in the duodenal portion. In the exocrine parenchyma of the splenic lobe, only insulin- and glucagon-IR cells were detected but all four kinds of IR cells were observed in the duodenal portion. In addition, insulin and hPP-IR cells were also demonstrated in the pancreatic duct regions. In conclusion, some strain-dependent characteristic distributional patterns of pancreatic endocrine cells were found in the ddN mouse with somewhat different distributional patterns between the two pancreatic lobes.
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About this article
Title

An immunohistochemical study of the pancreatic endocrine cells of the ddN mouse.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 48, No 3 (2010)

Pages

387-393

Published online

2010-11-13

DOI

10.2478/v10042-010-0026-y

Bibliographic record

Folia Histochem Cytobiol 2010;48(3):387-393.

Authors

Hyeung Sik Lee
Jeong Hyun Chang
Sae Kwang Ku

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