open access

Vol 48, No 4 (2010)
ORIGINAL PAPERS
Published online: 2011-04-12
Submitted: 2011-12-19
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The expression of B7-H1 and B7-H4 molecules on immature myeloid and lymphoid dendritic cells in cord blood of healthy neonates.

M Kludka-Sternik, A Serafin, D Darmochwal-Kolarz, S Radej, J Rolinski, B Leszczynska-Gorzelak, J Oleszczuk
DOI: 10.2478/v10042-010-0054-7
·
Folia Histochem Cytobiol 2010;48(4):658-662.

open access

Vol 48, No 4 (2010)
ORIGINAL PAPERS
Published online: 2011-04-12
Submitted: 2011-12-19

Abstract

The aim of our study was to estimate both B7-H1 and B7-H4 molecules on immature myeloid and lymphoid dendritic cells in umbilical cord blood of healthy neonates in comparison with peripheral blood of healthy adults. Thirty nine healthy full-term neonates from physiological single pregnancies and 27 healthy adults were included in the study. The expression of B7-H1 and B7-H4 was revealed using the immunofluorescence method. Statistical analysis was performed using a non-parametric test (Mann-Whitney U-Test). The percentages of BDCA-1+ dendritic cells with B7-H1 and B7-H4 expressions were significantly higher in peripheral blood of healthy adults (p<0.00003). It was either observed that the percentage of BDCA-2+ dendritic cells with the expression of B7-H4 molecules was significantly higher in peripheral blood of healthy adults in comparison with umbilical cord blood (p<0.02). Decreased percentages of dendritic cells and co-stimulatory molecules indicate that neonates have immature immune system. Depletion of co-stimulatory B7-H1 and B7-H4 molecules enable appropriate development of immune response.

Abstract

The aim of our study was to estimate both B7-H1 and B7-H4 molecules on immature myeloid and lymphoid dendritic cells in umbilical cord blood of healthy neonates in comparison with peripheral blood of healthy adults. Thirty nine healthy full-term neonates from physiological single pregnancies and 27 healthy adults were included in the study. The expression of B7-H1 and B7-H4 was revealed using the immunofluorescence method. Statistical analysis was performed using a non-parametric test (Mann-Whitney U-Test). The percentages of BDCA-1+ dendritic cells with B7-H1 and B7-H4 expressions were significantly higher in peripheral blood of healthy adults (p<0.00003). It was either observed that the percentage of BDCA-2+ dendritic cells with the expression of B7-H4 molecules was significantly higher in peripheral blood of healthy adults in comparison with umbilical cord blood (p<0.02). Decreased percentages of dendritic cells and co-stimulatory molecules indicate that neonates have immature immune system. Depletion of co-stimulatory B7-H1 and B7-H4 molecules enable appropriate development of immune response.
Get Citation
About this article
Title

The expression of B7-H1 and B7-H4 molecules on immature myeloid and lymphoid dendritic cells in cord blood of healthy neonates.

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 48, No 4 (2010)

Pages

658-662

Published online

2011-04-12

DOI

10.2478/v10042-010-0054-7

Bibliographic record

Folia Histochem Cytobiol 2010;48(4):658-662.

Authors

M Kludka-Sternik
A Serafin
D Darmochwal-Kolarz
S Radej
J Rolinski
B Leszczynska-Gorzelak
J Oleszczuk

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