Vol 48, No 4 (2010)
Original paper
Submitted: 2011-12-19
Published online: 2011-04-12
The evaluation of human papillomavirus and p53 gene mutation in benign and malignant conjunctiva and eyelid lesions.
Reszec Joanna, Zalewska Renata, Pepiński Witold, Skawronska Małgorzata, Piotr Bernaczyk, Lech Chyczewski
DOI: 10.2478/v10042-010-0081-4
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Folia Histochem Cytobiol 2010;48(4):530-533.
Vol 48, No 4 (2010)
ORIGINAL PAPERS
Submitted: 2011-12-19
Published online: 2011-04-12
Abstract
Papillomas and squamous cell carcinomas are the most common conjunctival and eyelid lesions. The etiology is still unclear and recently human papillomavirus infection and p53 gene mutation have been taken into consideration. The aim of our study was the evaluation of HPV DNApresence and p53 gene mutation in 45 benign and 38 malignant squamous lesions of the conjunctiva and eyelid. For HPV detection PCR-RFLP and immunohistochemical reaction were used; for p53 gene mutation PCR-SSCP was used. Only 8.8% papillomas, 9.1% squamous cell cancers and 3.7% basal cell cancers (using PCR-RFLP method) and 26.6% papillomas, 7.4% squamous cell cancers and 9.1% basal cell cancers (using immunohisto-chemical reaction) were HPV positive. p53 gene mutation was evaluated in 24.4% papillomas, 54.5% squamous cell cancers and 22.2% basal cell cancers; most commonly in 6 and 7 exon. Human papillomavirus infection, opposite to p53 gene mutation, is not a significant etiological factor of the benign and malignant conjunctival and eyelid lesions development.
Abstract
Papillomas and squamous cell carcinomas are the most common conjunctival and eyelid lesions. The etiology is still unclear and recently human papillomavirus infection and p53 gene mutation have been taken into consideration. The aim of our study was the evaluation of HPV DNApresence and p53 gene mutation in 45 benign and 38 malignant squamous lesions of the conjunctiva and eyelid. For HPV detection PCR-RFLP and immunohistochemical reaction were used; for p53 gene mutation PCR-SSCP was used. Only 8.8% papillomas, 9.1% squamous cell cancers and 3.7% basal cell cancers (using PCR-RFLP method) and 26.6% papillomas, 7.4% squamous cell cancers and 9.1% basal cell cancers (using immunohisto-chemical reaction) were HPV positive. p53 gene mutation was evaluated in 24.4% papillomas, 54.5% squamous cell cancers and 22.2% basal cell cancers; most commonly in 6 and 7 exon. Human papillomavirus infection, opposite to p53 gene mutation, is not a significant etiological factor of the benign and malignant conjunctival and eyelid lesions development.
Title
The evaluation of human papillomavirus and p53 gene mutation in benign and malignant conjunctiva and eyelid lesions.
Journal
Folia Histochemica et Cytobiologica
Issue
Vol 48, No 4 (2010)
Article type
Original paper
Pages
530-533
Published online
2011-04-12
Page views
2372
Article views/downloads
1817
DOI
10.2478/v10042-010-0081-4
Bibliographic record
Folia Histochem Cytobiol 2010;48(4):530-533.
Authors
Reszec Joanna
Zalewska Renata
Pepiński Witold
Skawronska Małgorzata
Piotr Bernaczyk
Lech Chyczewski