open access

Vol 51, No 1 (2013)
Original paper
Submitted: 2013-04-28
Accepted: 2013-04-28
Published online: 2013-04-24
Get Citation

Kainic acid induces expression of caveolin-1 in activated microglia in rat brain

Shigeko Takeuchi, Wakoto Matsuda, Ikuo Tooyama, Osamu Yasuhara
DOI: 10.5603/FHC.2013.004
·
Folia Histochem Cytobiol 2013;51(1):25-30.

open access

Vol 51, No 1 (2013)
ORIGINAL PAPERS
Submitted: 2013-04-28
Accepted: 2013-04-28
Published online: 2013-04-24

Abstract

Caveolin-1, a major constituent of caveolae, has been implicated in endocytosis, signal transduction and cholesterol transport in a wide variety of cells. In the present study, the expression of caveolin-1 was examined by immunohistochemistry in rat brain with or without systemic injection of kainic acid (KA). Caveolin-1 immunoreactivity was observed in capillary walls in brains of control rats. From one to seven days after KA injection, caveolin-1 immunoreactivity appeared in activated microglia in the cerebral cortex, hippocampus and other brain regions. The strongest immunoreactivity of microglia was seen after 3 days after KA administration. The expression of caveolin-1 was confirmed by RT-PCR and Western blot analysis, respectively. The induction of caveolin-1 expression in microglia activated in response to kainic acid administration suggests its possible role in a modulation of inflammation. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 25–30)

Abstract

Caveolin-1, a major constituent of caveolae, has been implicated in endocytosis, signal transduction and cholesterol transport in a wide variety of cells. In the present study, the expression of caveolin-1 was examined by immunohistochemistry in rat brain with or without systemic injection of kainic acid (KA). Caveolin-1 immunoreactivity was observed in capillary walls in brains of control rats. From one to seven days after KA injection, caveolin-1 immunoreactivity appeared in activated microglia in the cerebral cortex, hippocampus and other brain regions. The strongest immunoreactivity of microglia was seen after 3 days after KA administration. The expression of caveolin-1 was confirmed by RT-PCR and Western blot analysis, respectively. The induction of caveolin-1 expression in microglia activated in response to kainic acid administration suggests its possible role in a modulation of inflammation. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 25–30)

Get Citation

Keywords

caveolin 1 expression; brain; microglia; kainic acid; seizures; rat; immunohistochemistry

About this article
Title

Kainic acid induces expression of caveolin-1 in activated microglia in rat brain

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 51, No 1 (2013)

Article type

Original paper

Pages

25-30

Published online

2013-04-24

DOI

10.5603/FHC.2013.004

Bibliographic record

Folia Histochem Cytobiol 2013;51(1):25-30.

Keywords

caveolin 1 expression
brain
microglia
kainic acid
seizures
rat
immunohistochemistry

Authors

Shigeko Takeuchi
Wakoto Matsuda
Ikuo Tooyama
Osamu Yasuhara

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By "Via Medica sp. z o.o." sp.k., ul. Świętokrzyska 73, 80–180 Gdańsk

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl